|
|
Bioavailability of magnesium and zinc from food supplements
Lindovský, Patrik ; Křikala, Jakub (referee) ; Diviš, Pavel (advisor)
This bachelor's thesis deals with the analysis of dietary supplements containing magnesium and zinc. The analysis examined the content of active substance and solubility in simulated gastric acid. The method used to analyze the active substance content was inductively coupled plasma optical emission spectrometry. This method was also used to determine the degree of solubility of the active substances. A total of thirteen different samples of dietary supplements were examined, all of which contained magnesium as the active ingredient and four of these samples additionally contained zinc. All samples contained the declared amount of magnesium. The zinc amount in food supplements was in accordance with the content stated on the label. In some samples the amount of zinc was determined to be lower, but the deviation from the declared amount did not exceed 14 %. Solubility tests have shown that some products do not contain a suitable type of coating to protect the active substance, which may cause a deterioration in bioavailability.
|
|
|
Bioavailability of magnesium and zinc from food supplements
Lindovský, Patrik ; Křikala, Jakub (referee) ; Diviš, Pavel (advisor)
This bachelor's thesis deals with the analysis of dietary supplements containing magnesium and zinc. The analysis examined the content of active substance and solubility in simulated gastric acid. The method used to analyze the active substance content was inductively coupled plasma optical emission spectrometry. This method was also used to determine the degree of solubility of the active substances. A total of thirteen different samples of dietary supplements were examined, all of which contained magnesium as the active ingredient and four of these samples additionally contained zinc. All samples contained the declared amount of magnesium. The zinc amount in food supplements was in accordance with the content stated on the label. In some samples the amount of zinc was determined to be lower, but the deviation from the declared amount did not exceed 14 %. Solubility tests have shown that some products do not contain a suitable type of coating to protect the active substance, which may cause a deterioration in bioavailability.
|
|
|
Využití nanomateriálů pro zvýšení biodostupnosti doplňků stravy
Vašíčková, Kateřina
Bachelor thesis with the name Application of nanomaterials for enhancement of food supplements bioavailability deals with the effect of selected food supplements on human organism and the use of nanocarriers to improve their biological availability for organism. Literary part of this work is focused on the evaluation of problems of bioavailability of active food components. Then, the benefits and risks of taking food supplements are discussed, including model dietary supplements such as ascorbic and folic acids. The practical part of this work serves to evaluate the long-term stability of complex formed by protein nanocarrier (based on natural iron-storage protein apoferritin) with encapsulated molecules in various light conditions (ambient light, dark), temperatures (-20; 4; 20 and 37 C) and solvents (ACS water, PBS). Stability of this complex, the amount of prematurely released molecules and the size and shape of the nanocarrier were detected using absorbance and fluorescence of the encapsulated fluorescent tag, dynamic light scattering and transmission electron microscopy. The optimal storage conditions were dark, temperature of 4 C and ACS water as a solvent. Moreover, the capability of apoferritin to bind food supplements to apoferritin was researched. Different concentrations of ascorbic acid (615; 308; 154; 77 a 38 ug/ml) were successfully encapsulated to apoferritin when 39 % of all applied molecules were encapsulated. Folic acid was bonded on the surface of apoferritin in five different concentrations (200; 100; 50; 25 a 13 uM) using zero-length linker with 61 % efficiency. Finally, the stable complex of apoferritin with folic acid was applied to cellular line of neuroblastom UKF-NB4. It was discovered that higher concentration of encapsulated folic acid increased the amount of its internalization to cells. Therefore, the increase in bioavailability of folic acid and encapsulated molecules via nanocarriers was proved. The results of this work prove that the use of nanomaterials is applicable to food sector and at the same time can serve to improve the bioavailability of active substances during taking of dietary supplements.
|
guest ::
