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Laboratory diagnostics of kidney injury in patients with diabates mellitus I and II.
Múčková, Ľubica ; Dršata, Jaroslav (advisor) ; Tichý, Aleš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Ľubica Múčková Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Consultant: Ing. Jana Netriová, PhD. Title of diploma thesis: Laboratory diagnostics of kidney injury in patients with diabetes mellitus I and II Diabetes mellitus is a chronic metabolic disease. This disease leads to develompement of both microvascular and macrovascular complications. In this work we examined a microvascular complication - diabetic nephropathy - and its laboratory diagnostics. We focussed on parameters cystatin C and creatinine. We estimated a glomerular filtration with Grubb, Stevens and MDRD equations. The Grubb and Stevens equations use cystatin C to determine the glomerular fitrlation rate (GFR), MDRD equations use creatinine, albumin and urea. For the asessment of kidney function we evaluated albumin and total protein waste in urine samples collected for 24 hours. The parameters were measured in file of 183 patients. Number of pacients with diabetes I.type was 49 and with diabetes II.type was 134. The main intention of this work was to evaluate equations used for determination of GFR, to compare their pros and cons and put them in contrast with albumin and total proteins waste. In case of the patients...
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Laboratory diagnostics of kidney injury in patients with diabates mellitus I and II.
Múčková, Ľubica ; Dršata, Jaroslav (advisor) ; Tichý, Aleš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Ľubica Múčková Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Consultant: Ing. Jana Netriová, PhD. Title of diploma thesis: Laboratory diagnostics of kidney injury in patients with diabetes mellitus I and II Diabetes mellitus is a chronic metabolic disease. This disease leads to develompement of both microvascular and macrovascular complications. In this work we examined a microvascular complication - diabetic nephropathy - and its laboratory diagnostics. We focussed on parameters cystatin C and creatinine. We estimated a glomerular filtration with Grubb, Stevens and MDRD equations. The Grubb and Stevens equations use cystatin C to determine the glomerular fitrlation rate (GFR), MDRD equations use creatinine, albumin and urea. For the asessment of kidney function we evaluated albumin and total protein waste in urine samples collected for 24 hours. The parameters were measured in file of 183 patients. Number of pacients with diabetes I.type was 49 and with diabetes II.type was 134. The main intention of this work was to evaluate equations used for determination of GFR, to compare their pros and cons and put them in contrast with albumin and total proteins waste. In case of the patients...
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Influence of humic substance HS 1500 on tolerance of Sterlet to nitrite
BULÍČEK, Vojtěch
The aim of the thesis was to assess the effect of humic substance HS 1500 on the tolerance of Sterlet (Acipenser ruthenus) to nitrite. Preparation Huminfeed was used as a source of substance HS 1500. Tolerance of Sterlet to nitrite was assessed on the basis of the results of acute toxicity tests and the results of haematological and biochemical blood examination of fish that were exposed to increased concentrations of nitrite in the presence and absence of preparation Huminfeed.
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Comparison of level total cholesterol in serum for residents from Pilsen and Czech Budweis provided similar eating habits
FRAITOVÁ, Lucie
Measuring cholesterol is considered as a screening test used primarily to determine cardiovascular risk and monitoring lipid-lowering therapy. Since this is a screening examination, there is a need for further laboratory tests - the lipid profile (HDL cholesterol, LDL cholesterol, triglycerides). Patients who get examined as a part of lipid-lowering therapy should follow a diet. These patiens need to be examined more frequently. Population of Czech Republic is considered at risk of early occurrence of atherosclerosis and its complications. Every year in the Czech Republic nearly 60 000 people die from cardiac and vascular disease. Cardiovascular disease increasingly affects younger age group. They are also a cause of disability of people of working age. One of the main indicators is high level of cholesterol. Accurate and precise measurement of blood cholesterol plays a role in the reduction of morbidity and mortality of people with cardio vascular disease. The aim of this thesis is to: 1) Measure the total cholesterol sample population of Plzeň and České Budějovice 2) Compare and find differences in serum total cholesterol in both locations In the theoretical part of the thesis I was interested in what foods increase cholesterol and what foods can reduce high cholesterol. Furthermore, the theoretical part describes diseases that may occur during hypercholesterolemia. The basic ones include atherosclerosis and its complications, which include heart disease or stroke. Preanalytical factor that may significantly affect laboratory tests are described in this part too. The practical part describes everything from an intake of biological material in the laboratory through the preparation (centrifugation, creating aliquots) to insertion of a secondary sample into the analytical device. Research for the thesis was conducted in the biochemical laboratory of synlab czech s.r.o. in České Budějovice. In this part I investigate ? under supervision ? a total of 100 samples of venous blood from people of Plzeň and České Budějovice. I determined the total cholesterol in serum. Cobas Integra 800 automatic biochemical analyzer was used for the examination of the biological material. In routine practice enzymatic methods are used to determine levels of cholesterol. Cholesterol esters are converted by cholesterol esterase into free cholesterol and fatty acids. Free cholesterol is oxidized by cholesterol oxidase to form cholestenone and hydrogen peroxide. The resultant hydrogen peroxide reacts with 4-aminoantipyrine and phenol to form a red color and water. The color intensity is directly proportional to the concentration of cholesterol and is measured by absorption spectrophotometry at 512 nm.Measured laboratory results were statistically processed and neatly sorted into tables and graphs using the computer program. Data from Plzeň and České Budějovice were sorted according to gender and evaluated using graphs. The data sets were then compared to each other and the difference in cholesterol levels was registered. All the results are in the table and graphs. Hypercholesterolemia was detected in 29 samples from Plzeň and 27 from České Budějovice.
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Investigation of markers for the diagnostics of metabolic syndrome
KUNEŠ, Jan
In my Bachelor´s thesis I dealt with the issue of investigation of biochemical markers, which are used for diagnostics of metabolic syndrome along with measurement of waist circumference and level of blood pressure. In the theoretical part I defined the term metabolic syndrome. Chapters about glucose and blood lipids tests as well as methods used for their determination are described in detail. Great attention was also paid to preanalytical factors, which significantly share of the accuracy of laboratory test. Practical part of the Bachelor´s thesis is focused on acquisition of practical skills in work with biological material in the laboratory of clinical biochemistry and acquisition of principals of laboratory methods. Under professional supervision in the biochemical laboratory Laboma s.r.o. in České Budějovice, I tested 100 samples of venous blood, where I determined concentration of fasting glucose, triglycerides and HDL cholesterol. The measurement was carried out on the biochemical analyzer Cobas Integra 800 by the method of absorption spectrophotometry. Measured laboratory values were statistically processed and they were interpreted after consultation with the supervisor of the Bachelor´s thesis. The laboratory tests showed that the criteria of metabolic syndrome applied for 21% of women and 15% of men.
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