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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The safety of probiotics used in foods
 Balažovičová, Nikola ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
The theoretical part of this thesis focuses on definition of probiotics, their health benefits, probiotic foods, safety criteria for probiotics used in foods, including methods for the identification of probiotic bacteria. Experimental part of work was focused on the identification of probiotic bacteria contained in the milk product Actimel. DNA quality suitable for the PCR was isolated by magnetic microparticles P(HEMA-co-GMA). Identification was estimated using the method of polymerase chain reaction. The presence of DNA of domain Bacteria, genus Lactobacillus and species Lactobacillus casei was confirmed by gel electrophoresis of PCR products. Specific PCR products of the sizes of 466 bp (domain Bacteria), 250 bp (genus Lactobacillus) and 136 bp (Lactobacillus casei) were detected after amplification of DNA isolated from a probiotic milk product.
Detailed record
The safety of probiotics used in foods
 Balažovičová, Nikola ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
The theoretical part of this thesis focuses on definition of probiotics, their health benefits, probiotic foods, safety criteria for probiotics used in foods, including methods for the identification of probiotic bacteria. Experimental part of work was focused on the identification of probiotic bacteria contained in the milk product Actimel. DNA quality suitable for the PCR was isolated by magnetic microparticles P(HEMA-co-GMA). Identification was estimated using the method of polymerase chain reaction. The presence of DNA of domain Bacteria, genus Lactobacillus and species Lactobacillus casei was confirmed by gel electrophoresis of PCR products. Specific PCR products of the sizes of 466 bp (domain Bacteria), 250 bp (genus Lactobacillus) and 136 bp (Lactobacillus casei) were detected after amplification of DNA isolated from a probiotic milk product.
Detailed record

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