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Orphan Nuclear Receptor TLX (NR2E1) in Regulation of Cell Reproduction and Differentiation
Raška, Otakar ; Kostrouch, Zdeněk (advisor) ; Otová, Berta (referee) ; Nedvídková, Jara (referee)
Nuclear receptors constitute a large family of transcription factors that are powerful regulators of animal tissue metabolism, homeostasis, tissue maintenance and development. They are particularly attractive for their ability to respond to the binding of hormones, metabolites, xenobiotics and artificially prepared molecules and transmit the interaction with these small lipophylic molecules to specific regulatory potential. In search for nuclear receptors that are likely to be critical for neural tissues in invertebrates and conserved during the evolution of animals, we have identified a close homologue of vertebrate TLX in a planarian Schmidtea mediterranea. Planaria represent very promising biological model systems for studies on tissue maintenance and regeneration. Planaria are able to resorb their tissues and use them as sources of energy during fasting and they re-build their bodies from neoblasts when food is plentiful. Our search in Schmidtea mediterranea's publicly accessible genome sequencing data indicated that planarian genome contains at least one gene with a high degree of similarity to vertebrate TLX. We cloned full length CDS (coding DNA sequence of cDNA) and characterized the gene functionally. This showed that the planarian and vertebrate NR2E1 are highly similar...
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The results of treatment of primary brain tumours
MRÁZKOVÁ, Tereza
The study is aimed to primary brain tumour patient's prognosis. The study is comprised of data processing, basic information summary of the issue, evaluation of overall survival of primary brain cancer patients and results comparison to the literature. The hypothesis says that the results gained at department of oncology are comparable with the literature. Patient's data were assembled from medical history of Oncology ward of České Budějovice hospital. Data collection was aimed of gender, age, tumour localization, histology, WHO staging, way of treatment, date of treatment initiation and current condition. Study included 116 patients with treatment initiation between 2011 - 2015 at the Oncology ward of České Budějovice hospital. Data were processed using Excel and survival rate was assessed by Kaplan - Meier analysis of the statistical program IBM SPSS Statistics. Overall survival was assessed in terms of the way of treatment (a combination of resection and radiation therapy, a combination of resection, radiation therapy and chemotherapy, palliative treatment), histology (astrocytoma, oligodendroglioma, glioblastoma multiforme), gender and age (younger than 50 years old, older than 50 years). Median overall survival of whole group under treatment was 12 months. In terms of overall survival analysis based on the histological results and treatment (combinations of resection, radiation therapy and chemotherapy) was at variance with literature. The acquired results should serve to further processing by students and as feedback for the oncology ward, because it assesses success of treatment on department.
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Orphan Nuclear Receptor TLX (NR2E1) in Regulation of Cell Reproduction and Differentiation
Raška, Otakar ; Kostrouch, Zdeněk (advisor) ; Otová, Berta (referee) ; Nedvídková, Jara (referee)
Nuclear receptors constitute a large family of transcription factors that are powerful regulators of animal tissue metabolism, homeostasis, tissue maintenance and development. They are particularly attractive for their ability to respond to the binding of hormones, metabolites, xenobiotics and artificially prepared molecules and transmit the interaction with these small lipophylic molecules to specific regulatory potential. In search for nuclear receptors that are likely to be critical for neural tissues in invertebrates and conserved during the evolution of animals, we have identified a close homologue of vertebrate TLX in a planarian Schmidtea mediterranea. Planaria represent very promising biological model systems for studies on tissue maintenance and regeneration. Planaria are able to resorb their tissues and use them as sources of energy during fasting and they re-build their bodies from neoblasts when food is plentiful. Our search in Schmidtea mediterranea's publicly accessible genome sequencing data indicated that planarian genome contains at least one gene with a high degree of similarity to vertebrate TLX. We cloned full length CDS (coding DNA sequence of cDNA) and characterized the gene functionally. This showed that the planarian and vertebrate NR2E1 are highly similar...
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