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Effect of selected perfumes on aromatase activity
Drejslarová, Iva ; Hodek, Petr (advisor) ; Smrček, Stanislav (referee)
Cytochromes P450 are enzymes involved in many physiological processes in the body, besides the metabolism of xenobiotics also in the biosynthesis and catabolism of endogenous substances such as hormones and steroids. Cytochromes P450 that are responsible for the transformation of endogenous compounds can be affected by xenobiotics, which interfere with the secretion, metabolism, transport or elimination of endogenous hormones by various mechanisms. These substances are also referred to as endocrine disruptors and include, for example, pesticides, insecticides, certain pharmaceuticals, or compounds contained in cosmetic products. One of their mechanisms of action is to interfere with steroid hormone biosynthesis through interactions with steroidogenic enzymes. One such enzyme is cytochrome P450 19, aromatase, which catalyses the conversion of testosterone to ß-estradiol in the final step of steroid hormone biosynthesis. Modulation of the activity of this enzyme by endocrine disruptors results in an imbalance of estrogen levels in the body, which can lead to impaired reproduction, osteoporosis, atherosclerosis, dementia, and the development of certain types of cancer. In this context, the effect of commercially available perfumes on the conversion of testosterone to ß-estradiol, which is catalyzed by...
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Effect of selected cosmetic products on estrogen formation
Drejslarová, Iva ; Hodek, Petr (advisor) ; Smrček, Stanislav (referee)
Cytochrome P450 enzymes play a key role in the metabolism of endogenous and exogenous compounds. These enzymes are involved both in biotransformation and steroid hormone biosynthesis. An important member of this family is cytochrome P450 19, aromatase, which catalyzes the final step of estrogen hormone biosynthesis, conversion of androgens into estrogens. The physiologic functions of estrogens include development of secondary sexual characteristicts, maintenance of bone mass or regulation of gonadotropin secretion. However, hormonal imbalance due to endocrine disruptors can result in development of certain cancers or impaired reproduction. Compounds with these effects include, in addition to environmetal pollutants, some drugs and cosmetic additives which people are exposed to on a daily basis. In the present study, the effect of selected perfumes and antiperspirants on the metabolic conversion of testosterone into estradiol, catalyzed by aromatase, was examined. The activity of this enzyme was detected using TLC chromatography. The experiments showed that selected antiperspirants do not affect aromatase activity. For most of the perfumes tested, only a very low aromatase inhibition was observed. Only one sample showed this effect to a higher extent. Based on the comparision of perfume...
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Interaction of selected cosmetic additives with biotransformation enzymes
Bušovská, Radka ; Hodek, Petr (advisor) ; Smrček, Stanislav (referee)
Substances which cause an endocrine dysfunction have a negative impact on the human body and can be found all around us. One of the main mechanisms of their action is the binding to cell receptors or the inhibition of key enzymes involved in steroid hormone synthesis. In this study, the inhibitory effect of selected perfumes and car air fresheners on the activity of aromatase, the key enzyme of steroidogenesis, was examined. Aromatase converts male sex hormones into the female ones. Inhibition of this enzyme results in decreased estrogen concentrations and may thus affects human fertility. The aromatase activity was examined based on the aromatization reaction of testosterone into estradiol. The formation of estradiol was monitored by TLC method. Based on the results of the pilot study the tested perfumes do not significantly inhibit the aromatase activity, while air fresheners in cars appear to be potential aromatase inhibitors. Keywords: Endocrine dysfunction, steroidogenesis, aromatase, perfumes, air fresheners, TLC, chromatography
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Interaction of selected cosmetic additives with biotransformation enzymes
Bušovská, Radka ; Hodek, Petr (advisor) ; Smrček, Stanislav (referee)
Endocrine disruptors are nowadays very often discussed, due to their negative impact on the human body. They can be found all around us and they've become a parf of our everyday life, even if many people don't even realize it. Cosmetic products and perfumes are part of everyday life and just few of many people realize the consequences of having regular contact with our skin. Perfumes and fragrances are part of all cosmetic products or various commonly used industrial products, that people could not even live without. Perfumes can have a negative effect on the health of the organism and, due to the large number of chemicals they consist of and the interactions with each other and the subsequent interaction with important enzymes such as aromatase, they can belong to the group of hormone-active substances. The aim of this work was to find out by TLC (Thin Layer Chromatography) analytical method whether various perfumes and industrial car smells can disturb the endocrine system by inhibiting the key enzyme, aromatase. Experiments have shown that the commonly used fragrances really interact with aromatase. Keywords: Endocrine disruptors, aromatase, TLC, chromatography, perfumes, industrial fragrances
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