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Zkrmování pšenice Citrus u brojlerů
Karásek, Filip
This thesis deals with the finding of suitability for inclusion in the newly bred varieties of wheat Citrus with is , in higher lutein content in grain to the ration for fattening broilers. Influence on feed consumption, body weight gain, feed conversion ratio of broiler were observed. In the trial with male chickens Ross 308 were fed diet with 30 % and 60 % wheat as compared with control wheat. Effect of wheat Citrus to fattened broilers was not significant, but the relevance of the performance parameters influence wheat Citrus (P <0.05) was observed on the antioxidant activity using the method of free radicals (FR).
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Účinnost specifických aditiv ve výživě sportovních koní
Hanzlíková, Michaela
This thesis deals with the use of specific additives in the diet of sport horses. The theme is focused on the peppermint, and its antioxidant activity in horses. There is experiment conducted in 6 different horses to race, sex and age on antioxidant activity of superoxide dismutase SOD and GPx glutathione peroxidase. During the experiment was 4x took blood sample of horses to determine the activity of antioxidant enzymes in period of feeding without additives and feeding with activity use of additives in the diet. The results of experimental monitoring the activity of SOD, as well as in monitoring the activity of GPx enzyme revealed large individual differences between individual animals, which is typical for horses. In our attempt at SOD activity has not been demonstrated statistical relevance. Conversely, it was with the activity of glutathione, here, the results were statistically significant (P <0.05).
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Stanovení antioxidačního statusu u potkanů při zkrmování pšenice Citrus
Bendová, Kateřina
This thesis examines the influence of wheat Citrus with higher content of lutein in comparison with common wheat on antioxidant activity of the rat blood plasma and liver tissue. In the experiment, 32 male rats of Wistar strain were used. The influence of the feeding of yellow wheat varieties Citrus on antioxidant activity was measured in the blood and liver tissue. The experimental group (N = 16) was fed with dried granules of 100% wheat meal from Citrus. The control group (N = 16) was fed with similar feed prepared from two common wheat. Content of crude protein was the same in the both group. Body weight gain were followed in three-day intervals and feed consumption was followed daily. Antioxidant activity was measured by DPPH, FR, FRAP and ABTS methods. Values are expressed in gallic acid equivalent. In the blood significant differences (P < 0.05) of antioxidant activity was determined only by ABTS method between "Citrus" group (405.51 +- 7.64 mg /ml) in comparison to "Control" group (479.99 +- 22.54 mg/ml) in contrary to expectation this value of control group was of 18% higher. Values of other antioxidant methods were not significant. In the liver tissue only average value of ABTS method was little higher in Citrus group (409,439 +-13,626) in comparison with control group (389,493 +- 9,271 mg/ml). Similar situation was with DPPH method (20.711 +- 0.867 in Citrus group and 20.037 +- 1.109 mg/ml in the Control group) and difference was also not significant (P> 0.05).
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Effect of enzyme hydrolysis on solubility and antioxidative properties of potato protein isolates derived from heat coagulation
MIKOVÁ, Klára
The bachelor thesis is focused on effect of enzyme hydrolysis on solubility and antioxidative properties of potato protein. It was used industrial potato fruit juice and potato fruit juice from tubers two varieties Sibu and Ornella. Isolates derived from heat coagulation are generally poorly soluble so their use is limited (only feeding purporses). This work describes positive effect of enzyme hydrolysis on solubility and antioxidative properties of potato protein isolates. For example, antioxidative activity of soluble isolates was 99 mg of L - ascorbis acic per mg grifts. The highest share of soluble isolates was found at cleaved of enzyme alkalasa
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The Methods for the Detection of Antioxidant Substances
TURKOVÁ, Kateřina
Antioxidants and free radicals are widely discussed in the clinical and nutritional literature. Various reactive oxygen species (ROS) may be produced from normal biochemical, essential metabolic processes or from external sources as exposure to a variety of agents presented in the environment. Lipids, proteins, carbohydrates and DNA are all capable of reacting with ROS and can be implicated in etiology of various human disorders. The antioxidant system protects the organism from the effects of free radicals by scavenging them and thus preventing structural damage of cells. Measuring the individual components of the antioxidant system has provided important information about the defences against free radical effect. During the last decade, many analytical methods have been developed to determine the antioxidant activity of natural compounds and their mixtures in vitro. Some procedures involve the methods based on the generation of various radical species and their elimination by treatment with potential antioxidant compounds. The tests used for the evalution of total antioxidant activity are: Trolox equivalent antioxidant capacity-TEAC, diphenylpicrylhydrazil - DPPH, oxygen radical absorbance capacity-ORAC and the methods testing the ability to prevent lipid peroxidation. The other group includes the procedures that are based on measurement of redox properties of compouds (ferric reducing antioxidant potential assay-FRAP, cyclic voltammetry, HPLC).
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