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Pre-transfusion laboratory examination of patients with AIHA and multiple myeloma
INGRIŠOVÁ, Karolína
This bachelor thesis follow up laboratory pre-transfusion tests in the special imunohematology lab for patients with AIHA diagnosis and multiple myeloms. For said patients, compatibility of the erythrocyte tranfusion must be maintained using special procedures. For AIHA patients, that means removal of patient's auto-antibodies by saturating the patient's plasma sample. As for patients with multiple myeloma, treatment of erythrocytes (either diagnostic or donor) with DTT is needed to remove false positivity caused by other treatments (such as administration of Daratumumab). Theoretical part consists of explanation of the basic immunohematological terms followed by thorough description of tests needed for patients with mentioned problematic diagnosis. Practical parts describes all laboratory procedures of each individual pre-transfusion tests including special ones for patients with AIHA and MM. This part also contains evaulation and analysis of all patients with AIHA and MM examined at the transfusion department of the University Hospital in Pilsen during the years 2021 and 2022. At the end of this part, all aquired data are evaluated using charts and graphs. End of this thesis contains summary and importance of tests performed for patients with the diagnosis of AIHA and MM, including answers for previously set research questions.
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Reakce imunitního systému na spirochéty Lymské boreliózy v závislosti na jejich patogenním potenciálu
ŠMÍDOVÁ, Hana
An infectious disease known as Lyme borreliosis is caused by the bacterium Borrelia, which is transmitted to humans by ticks. The bachelor thesis deals with the comparison of the pathogenic potential of three species of bacteria from the Borrelia burgdorferi sensu lato complex, which are characterized by their antigenic equipment. Differences between these species result in different responses of the host immune system and also affect tissue specificity, it determines the target area in the body where a given bacterium persists and causes symptoms typical of its species.
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Salivary glycoproteins of bloodsucking arthropods
Sumová, Petra ; Volf, Petr (advisor) ; Mikeš, Libor (referee)
During obtaining their blood meal, bloodsucking arthropods salivate into their host. Bloodsucking arthropods' saliva contains wide array of bioactive macromolecules. Host organism develops antibody response against many of these molecules. Due to interspecies variability in salivary protein composition, detection of antibody response may serve as a marker of the exposure to individual species of bloodsucking arthropods. Host antibody response is mostly elicited by proteins or glycoproteins. Glycoproteins contain one or more oligosaccharide chains attached to the protein. Glycoprotein's antigenicity could be caused by either both parts, or by only the protein, or the sugar part. This fact has to be taken into consideration for choice of the expression system for recombinant glycoprotein synthesis. This work summarizes current knowledge about structure, function and features of salivary glycoproteins in various species of bloodsucking arthropods.
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Salivary glycoproteins of bloodsucking arthropods
Sumová, Petra ; Volf, Petr (advisor) ; Mikeš, Libor (referee)
During obtaining their blood meal, bloodsucking arthropods salivate into their host. Bloodsucking arthropods' saliva contains wide array of bioactive macromolecules. Host organism develops antibody response against many of these molecules. Due to interspecies variability in salivary protein composition, detection of antibody response may serve as a marker of the exposure to individual species of bloodsucking arthropods. Host antibody response is mostly elicited by proteins or glycoproteins. Glycoproteins contain one or more oligosaccharide chains attached to the protein. Glycoprotein's antigenicity could be caused by either both parts, or by only the protein, or the sugar part. This fact has to be taken into consideration for choice of the expression system for recombinant glycoprotein synthesis. This work summarizes current knowledge about structure, function and features of salivary glycoproteins in various species of bloodsucking arthropods.
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