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Vliv praziquantelu na raná vývojová stádia amura bílého
BÁRTOVÁ, Lucie
Praziquantel belongs to a group of medicines called anthelmintics, which are used to control parasitic infections. The aim of this study was to assess the effect of praziquantel on hatching, mortality, ontogenetic development, growth, oxidative stress and antioxidant enzyme activity in the early life stages of grass carp (Ctenopharyngodon idella). To assess the toxicity of praziquantel for the early life stages of grass carp, an embryo-larval fish toxicity test (OECD test no. 210) was used. The toxic effects of praziquantel were investigated when exposed to four concentrations (1 mg/l, 2 mg/l, 4 mg/l, 6 mg/l) for 29 days. A negative effect on curling was not observed in any group. A negative effect on mortality was observed in fish exposed to the concentrations of praziquantel 2 mg/l, 4 mg/l and 6 mg/l. Groups exposed to praziquantel concentrations of 4 mg/l and 6 mg/l died during the test period. A negative effect on ontogenetic development was observed in fish exposed to praziquantel concentrations of 4 mg/l and 6 mg/l. A negative effect of praziquantel on the activity of glutathione-S-transpherase was observed in the group exposed to a praziquantel concentration of 2 mg/l. A concentration of 1 mg/l praziquantel can be considered as a safe concentration for the early life stages of grass carp in the case of long-term exposure.
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Hematologické vyšetření ryb po antiparazitární léčbě
JANÍK, Jan
The aim of this bachelor's thesis was to consider the effect of chosen anti-parisitics on hematological indicators in the blood of fish and consequently study the effect of this medicaments on parasitic disease of lens caused by tremato-da Diplostomum spathaceum. Grass carps infected with trematoda were exposed to broad-spectrum anti-parasitics - praziquantel for 24 hours in the concentrations 2 mg.l-1 and 10 mg.l-1 and also in the benzimidazole derivatve - fenbendazole in the concentration 25 mg.l-1. Afterwards blood was taken from the fish and following indicators were stated - red blood cell (RBC), white blood cell (WBC), hematocrit (PCV), hemo-globin (Hb), mean corpuscural volume (MCV), mean corpuscural hemoglobin (MCH), mean corpuscural hemoglobin concentration (MCHC). Another step was to examine the lens and consider the effect of medicaments on metacercariae of Diplostomum spathaceum. Based on the results it was clear that there was a statistical decrease in the amount of erythrocytes (p<0.05) when using fenbendazole in the concentration 25 mg.l-1. Furthermore the results showed that when the concentration of pra-ziquantel was higher (10 mg.l-1), there was considerable increase in the middle colored concentration (p<0.05). The other indicators did not differ from the pa-rameters of the control groups. This examination showed only small statistically insignificant aberration, which was given by the low initial prevalence of Diplostomum spathaceum in the examined fish.
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Comparison of survival, growth rate and overall breeding of grass carp (Ctenopharyngodon idella) juveniles in pond and recirculating aquaculture system during winter time.
PECHER, Petr
The study compared survival and growth rate in grass carp (Ctenopharyngodon idella) juveniles in pond and recirculation aquaculture system (RAS) during winter time. The juveniles of grass carp were stocked to 5 tanks and three ponds during 2013 and 2014. In the RAS, the fish were fed by dry pellets and rearing was in constant temperature. In the pond, the temperature and feeding were only natural. In the RAS, the survival rate was significantly higher 97.79 % 0.52 % compared to 10.94 % 11.42 % in the pond. At the end of experiment, the weight of fish, Specific growth rate (SGR) and Fulton coefficient (FK) was significantly higher in RAS in comparison with the pond. The results showed that the rearing of grass carp is more effective in RAS in comparison with pond during winter time.
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