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Significantion of sumoylation for infection by selected viruses replicated in the cell nucleus
Sejpková, Marie ; Forstová, Jitka (advisor) ; Frydrýšková, Klára (referee)
This work introduces association between viruses and host cell with respect to sumoylation process. The main aim is referring to influence of this modification both on virus replication strategy and cell cycle. Sumoylation is essential process for cell regulation interfering with general regulation pathways including those performed by e.g. p53 or PML bodies and also epigenetic changes of chromatin. For viruses, sumoylation means stabilization of viral proteins and better timing each phase of viral cycle through viral protein. One point of view is competition of cell and virus for SUMO machinery. Viruses take advantage of sumoylation for inhibition antiviral defense of cells, regulation cell cycle mainly in apoptosis induction and in general for more successful infection. There are cumulating evidence of new proteins and phenomena connected with sumoylation mechanisms as well as viruses exploiting sumoylation for their benefit. Utilization and abuse of sumoylation by viruses point to future possibilities of cell manipulation and virus ability to intervene to this still relatively poorly understood type of cell regulation.
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Significantion of sumoylation for infection by selected viruses replicated in the cell nucleus
Sejpková, Marie ; Forstová, Jitka (advisor) ; Frydrýšková, Klára (referee)
This work introduces association between viruses and host cell with respect to sumoylation process. The main aim is referring to influence of this modification both on virus replication strategy and cell cycle. Sumoylation is essential process for cell regulation interfering with general regulation pathways including those performed by e.g. p53 or PML bodies and also epigenetic changes of chromatin. For viruses, sumoylation means stabilization of viral proteins and better timing each phase of viral cycle through viral protein. One point of view is competition of cell and virus for SUMO machinery. Viruses take advantage of sumoylation for inhibition antiviral defense of cells, regulation cell cycle mainly in apoptosis induction and in general for more successful infection. There are cumulating evidence of new proteins and phenomena connected with sumoylation mechanisms as well as viruses exploiting sumoylation for their benefit. Utilization and abuse of sumoylation by viruses point to future possibilities of cell manipulation and virus ability to intervene to this still relatively poorly understood type of cell regulation.
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Testing of biological materials in surgeries of pediatricians - POCT
FRDLÍKOVÁ, Adéla
This thesis is concerned with the accumulation of results of the POCT diagnostic methods in a pediatrician's office and the results of measurements in a respective laboratory. I examined and gathered the results of three mostly tested biological materials which are blood, urine and stool. For the urinalysis, I conducted a basic chemical, microscopic and microbiological examination. When doing blood analysis, I determined the amount of C-reactive protein in blood. For stool analysis, I checked for the presence of rotaviruses and adenoviruses. The general description of the individual examinations is to be found in the theoretical part of this thesis. In methodology, I described the apparatus and methods in detail. To chemically analyze the urine, I worked with HarnUReader device which operates on the principle of photometrics. In a laboratory, I checked the provided results against HeptaPhan diagnostic strips and a microscope analysis. The presence of bacteria in the urine was established in the pediatrician's office by using the Uricult Trio cultivation tests, which are coated with a layer of agar on both sides. I confirmed these results in a laboratory by a bacterial inoculation on blood agar and Endo soil. In the pediatrician's office, I used the Quikread device which measures the amount of C-reactive protein in the specimen on the principle of photometrics. In the laboratory, I checked these results against the results made by the fully automated Architest plus c4000 device which works on the principle of turbidimetry. To detect the presence of rotaviruses and adenoviruses in the stool, I used the Diarlex MB set in the pediatrician's office and the Rapid-VIDITEST Rota Adeno set in the laboratory. This thesis accurately describes the course of action I took to work with the respective methods and explains the detected results. The results of my work in the pediatrician's office and in the laboratory are summarized in the discussion.
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