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Optimization of bioethanol production by Zymomonas mobilis
Andrlová, Kateřina ; Vítová, Eva (referee) ; Babák, Libor (advisor)
Diploma thesis deals with use of Zymomonas mobilis for the production of bioethanol from waste paper. There were used three kinds of substrate (cardboard, drawing and office paper) to optimize of bioethanol production. Individual papers were subjected to the same pre treatment, namely a milling, a combination of microwave irradiation and NaOH, a combination of microwave irradiation and H2SO4 and combination microwave irradiation, H2SO4 and NaOH. The substrates were decomposed by enzymatic hydrolysis after pre treatment to evaluate the best pre-treatment. Simultaneous saccharification and fermentation was carried out for each substrate (with two of the best pre-treatment). The samples were taken during the hydrolysis and the simultaneous saccharification and fermentation, and were determined by HPLC. Growth curves of Zymomonas mobilis were constructed, as the most appropriate for SSF was chosen temperature of 40 ° C in which the exponential phase took place at the time of 6 15 hours. During hydrolysis was monitored glucose concentration in the solution. The maximum concentration of glucose was in the cardboard (microwaves + H2SO4 + NaOH) 16.46 gdm-3, a drawing (microwaves + H2SO4 + NaOH) 31.78 gdm-3, and office paper (microwaves + H2SO4) 25.04 gdm-3. The concentration of ethanol for SSF was highest in the same cases as in the hydrolysis. The cardboard was the maximum concentration of bio ethanol 9.5 gdm-3, for the drawing 16.1 gdm-3 and for the office paper 12.13 gdm-3.
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Application of Zymomonas mobilis for bioethanol production from cellulose materials
Čalová, Iveta ; Zichová, Miroslava (referee) ; Babák, Libor (advisor)
The thesis deals with the use of Zymomonas mobilis to produce bioethanol from waste paper. There were used three types of paper made by various manufacturers to optimize the production of bioethanol. Individual papers were subjected to the same adjustments namely mechanical grinding and subsequent enzymatic hydrolysis. During cultivation of Zymomonas mobilis in different hydrolysates were taken samples for determination of bioethanol production. The analysis was carried out on high-performance liquid chromatography. Production of bioethanol and glucose consumption was evaluated as well as production of lactic acid since it was also noticeable on the chromatograms.
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Optimization of bioethanol production by Zymomonas mobilis
Andrlová, Kateřina ; Vítová, Eva (referee) ; Babák, Libor (advisor)
Diploma thesis deals with use of Zymomonas mobilis for the production of bioethanol from waste paper. There were used three kinds of substrate (cardboard, drawing and office paper) to optimize of bioethanol production. Individual papers were subjected to the same pre treatment, namely a milling, a combination of microwave irradiation and NaOH, a combination of microwave irradiation and H2SO4 and combination microwave irradiation, H2SO4 and NaOH. The substrates were decomposed by enzymatic hydrolysis after pre treatment to evaluate the best pre-treatment. Simultaneous saccharification and fermentation was carried out for each substrate (with two of the best pre-treatment). The samples were taken during the hydrolysis and the simultaneous saccharification and fermentation, and were determined by HPLC. Growth curves of Zymomonas mobilis were constructed, as the most appropriate for SSF was chosen temperature of 40 ° C in which the exponential phase took place at the time of 6 15 hours. During hydrolysis was monitored glucose concentration in the solution. The maximum concentration of glucose was in the cardboard (microwaves + H2SO4 + NaOH) 16.46 gdm-3, a drawing (microwaves + H2SO4 + NaOH) 31.78 gdm-3, and office paper (microwaves + H2SO4) 25.04 gdm-3. The concentration of ethanol for SSF was highest in the same cases as in the hydrolysis. The cardboard was the maximum concentration of bio ethanol 9.5 gdm-3, for the drawing 16.1 gdm-3 and for the office paper 12.13 gdm-3.
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Application of Zymomonas mobilis for bioethanol production from cellulose materials
Čalová, Iveta ; Zichová, Miroslava (referee) ; Babák, Libor (advisor)
The thesis deals with the use of Zymomonas mobilis to produce bioethanol from waste paper. There were used three types of paper made by various manufacturers to optimize the production of bioethanol. Individual papers were subjected to the same adjustments namely mechanical grinding and subsequent enzymatic hydrolysis. During cultivation of Zymomonas mobilis in different hydrolysates were taken samples for determination of bioethanol production. The analysis was carried out on high-performance liquid chromatography. Production of bioethanol and glucose consumption was evaluated as well as production of lactic acid since it was also noticeable on the chromatograms.
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