|
|
The parasitic microorganisms in immunodeficient and healthy population of honebees (Apis mellifra)
Bičianová, Martina ; Hubert, Jan (advisor) ; Titěra, Dalibor (referee)
Immunodeficient honey bee (Apis mellifera) colonies suffer from broad range of parasites including eukaryotic protozoa. Despite this fact, the eukaryotic parasites are still poorly documented in the Czech Republic. The presence of eukaryotic parasites (Nosema ceranae, Nosema apis, Crithidia mellificae and Apicystis bombi) was observed in different apiaries in the Czech Republic. The samples were taken in 9 apiaries in 53 beehives during the 2014/2015 season. From each beehive, 10 adult of honey bees were taken from the peripheral comb in triplicate. DNA was isolated from every sample of honey bees. The parasites were detected by polymerase chain reaction (PCR) with specific primers. The treatment fall of parasitic mite Varroa destructor was obtained from beekeepers for season of 2014. Crithidia mellificae was detected by 5 types of specific primers (SEF, SER; SSU, SSU rRNA, Cyt b, Tryp cyt b) and positive amplicons were cloned and sequenced. The obtained sequences were compared with GeneBank and showed similarity from 98-100% to sequences of Lotmaria passim (Trypanosomatid). Crithidia mellificae was not detected. L. passim had prevalence of 79,2% and is reported in the Czech Republic for the first time. Primer Tryp-cyt b is recommended for the routine detection of L. passim. Nosema ceranae was...
|
|
| |
|
|
Study of physiology and searching of proteomic tools useful in suppression and detection of Varroa destructor
Holenková, Martina ; Erban, Tomáš (advisor) ; Titěra, Dalibor (referee)
This work is focused on the study of physiology and proteome of the the mite Varroa destructor and on comparison with the honeybee Apis mellifera. Varroa is currently a major problem for beekeeping, because infects most of the colonies. The control of the mite can not be done without residues both in the hive and for example in the honey or other resources used by the man. Another problem can also be the simultaneously discussed issue of the connection with the Colony Collapse Disorder. The internal anatomy of V. destructor was studied by using paraffin histology. On histological sections stained using hematoxylin and PAS was possible to observe the mite digestive system, but also egg, ovaries or brain. The primary aim of this study was to identify the proteins of mite V. destructor and bee A. mellifera as a host of this parasite. For the electrophoretic separation was used two- dimensional gel electrophoresis, where the second dimension was carried out using 12% and 15% SDS-PAGE. The most abundant spots were selected for analysis using MALDI TOF/TOF mass spectrometry. The most abundant protein identified in samples of V. destructor was hexamerin, arginine kinase or hemelipoglycoprotein precursor. Hexamerins were also identified as the major proteins in the pupae samples. On the contrary the main...
|
|
|
Influence of the protectives against Varroa destructor on the colonies of bees and the environment
Marečková, Kateřina ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
Varroa destructor contains about six different types of mites that came from Asia and spread to Europe and gradually to the entire world. In the 70‘s of the last century the mite was identified also in the Czech Republic. The danger of the Varroa destructor is that it‘s getting into the drone´s fetus, where it is closed by the bees. Herein the mite is procreated and developed. Since 1990, the number of colonies of bees in the Czech Republic has been decreased. This is because of various reasons but one of the main causes is varroa destructor. Correct treatment of bees is directed by the regulation number 2/2007 which was released by the regional administration for South Moravia. The regulation recommends the spring, summer, autumn and winter treatment of bees by the preparations M-1 AER, FORMIDOL laminaes, GABON PA 92 and PF 90 and VARIDOL FUM and AER. All the preparations except FORMIDOL are available only on medical prescription and they are manufactured in the Bee Research Institute at Dol (Libčice nad Vltavou). This work reviews the information on the mite Varroa destructor, his origin, distribution, therapeutics and prevention. It explores the influence of the preparations used in the Czech Republic on the environment and on the bees themselves.
|
|
|
Dignostika varroózy a možnosti selekce včely medonosné na varroatoleranci
Lukášek, Dominik
This work deals with the varroose of honey bees, its Diagnostics, control and the possibility of the formation of the natural resistance of the beehives. Here is described the evolution of mite and its weak spots in the reproduction. Then there are described the various methods of estimation of contamination by the presence of Varroa, including their benefits and pitfalls. Another part of the work is devoted to each of the factors responsible for the natural resistance of the hives to varroose. This is primarily about the active removal of contested the fetus. VSH behavior and active elimination of mites from their own bodies and hive environment known as grooming. Important is also the birthing colony mode and the ratio of worker bees and drone of the fetus. In the work are briefly characterized by chemical control measures, with an emphasis on organic acids. In the practical part of evaluating the fallout, washes off and sanitary tests carried out at University honey bee Apiary. The subsequent discussion deals with the utility of the individual methods Varroatolerance and estimate the adequacy of their use in practice.
|
guest ::
