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Species concept in the genus Trentepohlia (Ulvophyceae, Chlorophyta): a combination of molecular and morphological approaches
Šafránková, Michala ; Škaloud, Pavel (advisor) ; Hodač, Ladislav (referee)
In this Master's thesis I engage in the problematics of the species concept of green subaerial alga Trentepohlia (Ulvophyceae, Chlorophyta). This genus has been studied intensively since the 18th century. For more than 200 years, Trentepohlia species have been delimited using various morphological criteria. However, recent molecular studies showed inadequacies in this approach Therefore, my goal was a precise morphological study of the European Trentepohlia species and the re-evaluation of their phylogenetic position using rbcL gene sequencing. During the period 2013-2015, I carried out a detailed study of the oldest references of Trentepohlia species and compiled a delimitation key of the European species. Based on this research I concluded that it would be desirable to synonymize T. odorata with T. jolithus, as well as T. uncinata with T. arborum. I also sampled Trentepohlia species within Europe and carefully studied and described their morphology. Fresh Trentepohlia thalli were molecularly characterized by cloning, which revealed a common mixture of Trentepohlia species in what on a first sight appears to be a homogenous crust. Phylogenetic analyses based on the rbcL confirmed the ongoing inconsistencies among morphologically and molecularly delimited species and also the existence of cryptic...
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Species concept in the genus Trentepohlia (Ulvophyceae, Chlorophyta): a combination of molecular and morphological approaches
Šafránková, Michala ; Škaloud, Pavel (advisor) ; Hodač, Ladislav (referee)
In this Master's thesis I engage in the problematics of the species concept of green subaerial alga Trentepohlia (Ulvophyceae, Chlorophyta). This genus has been studied intensively since the 18th century. For more than 200 years, Trentepohlia species have been delimited using various morphological criteria. However, recent molecular studies showed inadequacies in this approach Therefore, my goal was a precise morphological study of the European Trentepohlia species and the re-evaluation of their phylogenetic position using rbcL gene sequencing. During the period 2013-2015, I carried out a detailed study of the oldest references of Trentepohlia species and compiled a delimitation key of the European species. Based on this research I concluded that it would be desirable to synonymize T. odorata with T. jolithus, as well as T. uncinata with T. arborum. I also sampled Trentepohlia species within Europe and carefully studied and described their morphology. Fresh Trentepohlia thalli were molecularly characterized by cloning, which revealed a common mixture of Trentepohlia species in what on a first sight appears to be a homogenous crust. Phylogenetic analyses based on the rbcL confirmed the ongoing inconsistencies among morphologically and molecularly delimited species and also the existence of cryptic...
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Molecular diversity and species concept of subaerial green alga Trentepohlia (Ulvophyceae, Chlorophyta)
Šafránková, Michala ; Škaloud, Pavel (advisor) ; Štifterová, Anna (referee)
This Bachelor thesis discusses green subaerial algae of the genus Trentepohlia (Ulvophyceae, Chlorophyta). Firstly, I focus on general description of the family Trentepohliaceae and the genus Trentepohlia, especially on morphology, ultrastructure and reproduction. Secondly, I summarize the knowledge of the literature available about the species of the genus Trentepohlia living in the Czech Republic. I also concern with the symbiotic relationship of these algae with other organisms and with some curiosities of their growth leading to ecological and other important consequences. Finally, in this thesis I introduce the issues of the species delimitation using traditional morphological methods in contrast to molecular approaches. There are several facts resulting from this thesis. The genus Trentepohlia is a green alga of the Chlorophyta lineage curious in some aspects, mostly due to presence of some structures among the cells (such as plasmodesmatas, MLS, carotenoids, etc.). There is also a problem of determination of individual species. For almost two hundred years, this problem has been solved by use of morphological features. However, molecular methods are often in contrast to these traditional methods. Therefore, reorganization on a genus level is essential, based on the discovery of...
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