|
|
Detecting Objects in Images
Kubínek, Jiří ; Beran, Vítězslav (referee) ; Hradiš, Michal (advisor)
This work is dedicated to methods used for object detection in images. There is a summary of several approaches and algorithms to solve this matter, especially AdaBoost algorithm with its improvement, WaldBoost and several features used for object detection. Vital part of this work is dedicated to extending training datasets for classifier training and extending the current object detection framework with histogram of gradients features implementation. Integral part of this work is analysis of results by experiments evaluation.
|
|
|
Utilization of protein radical foootprinting for stuctural biology
Polák, Marek ; Novák, Petr (advisor) ; Junková, Petra (referee)
(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and MS-based techniques, respectively. Optimal experimental conditions were achieved to oxidize the protein itself and in the presence of DNA, respectively. Oxidized samples were analyzed by bottom-up and top-down approach. In the bottom-up experiment, modification of individual residues was precisely located and quantified. Different extend of modification was observed for protein alone and in complex with DNA. To avoid experimental artifacts analyzing multiply oxidized protein, standard bottom up approach was replaced by a progressive top-down technology. Only a singly oxidized protein ion was isolated, and further fragmented by collision-induced dissociation (CID) and electron-capture dissociation (ECD),...
|
|
|
Utilization of protein radical foootprinting for stuctural biology
Polák, Marek ; Novák, Petr (advisor) ; Junková, Petra (referee)
(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and MS-based techniques, respectively. Optimal experimental conditions were achieved to oxidize the protein itself and in the presence of DNA, respectively. Oxidized samples were analyzed by bottom-up and top-down approach. In the bottom-up experiment, modification of individual residues was precisely located and quantified. Different extend of modification was observed for protein alone and in complex with DNA. To avoid experimental artifacts analyzing multiply oxidized protein, standard bottom up approach was replaced by a progressive top-down technology. Only a singly oxidized protein ion was isolated, and further fragmented by collision-induced dissociation (CID) and electron-capture dissociation (ECD),...
|
|
|
Landscape planning study as a tool for physical planning
Masojídková, Petra ; Perlín, Radim (advisor) ; Šantrůčková, Markéta (referee)
The diploma thesis focuses on the new tool Landscape planning study for the municipality with extended competence. The main aim of this work is to find out, how Landscape planning study can be used, who can use it and how it may change the landscape. The thesis uses the case study methods of three selected municipalities with extended competence, namely Votice, Humpolec and Vodňany. The theoretical part discusses the concept of landscape and anchoring in planning activities. In next part, the thesis deals with the basic forms of tools that regulate the planning and using of landscape in Czechia, focusing mainly on spatial planning. Furthermore, the thesis deals with models that currently affect space planning, namely top-down and bottom-up models. Two basic hypotheses of the diploma thesis are set based on theoretical frameworks. There are two qualitative research methods used in the practical part of the thesis. The first method is the analysis of Landscape planning studies, second method are semi-structured interviews with selected respondents. The results of both methods show that Landscape planning study can be used only to a limited extent, mainly as an information source. However, the implementation rate of specific measures is very low now. The identified causes are the lack of...
|
|
|
Detecting Objects in Images
Kubínek, Jiří ; Beran, Vítězslav (referee) ; Hradiš, Michal (advisor)
This work is dedicated to methods used for object detection in images. There is a summary of several approaches and algorithms to solve this matter, especially AdaBoost algorithm with its improvement, WaldBoost and several features used for object detection. Vital part of this work is dedicated to extending training datasets for classifier training and extending the current object detection framework with histogram of gradients features implementation. Integral part of this work is analysis of results by experiments evaluation.
|
guest ::
