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Genetická analýza zbarvení u huculských koní zařazených do genetického zdroje
Karbusická, Alžběta
In this work, MC1R, ASIP and TBX3 gene were tested on a sample of 118 Hucul horse mares included in Genetic Resources of Animals in the Czech Republic. We want to determine the genetic structure of mares and to analyse phenotypic data compared to the genotype and to identify possible differences between it. Genetic analysis showed a solid state for all alleles (HW for ASIP P = 0.9360, for MC1R P = 0.1661 and for TBX3 P = 0.4444). The frequency of the allele was as follows: E (0.6780), A (0.5254), and (0.4746), d2 (0.4323), d1 (0.3542), e (0.3220) D (0.2135). The most common genotype was AaEed1d2 and AaEEd1d2. There were very few or no genotypes based on recessive homozygotes in the genes of basic coat colours in the population, we didn´t identify any individual with genotype AaEed1d1. We have publicised genotype dependence within the TBX3 gene with primitive markings, confirming the previous work of other. Alele D was always associated with the occurrence of primitive markings, but primitive markings occur even without allele D in coincidence with the d1 allele. The d2d2 genotype is associated with a phenotype without primitive markings, or with phenotype where we can´t say if the horse has primitive markings or not.
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