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Sequential fractionation of organic matter of humic acid isolated from Leonardite
Krist, Tomáš ; Doskočil, Leoš (referee) ; Enev, Vojtěch (advisor)
The aim of the diploma thesis was to optimize the sequential fractionation method of organic matter to be used for physico-chemical characterization of extracted fractions. Humic acid isolated from oxidized brown coal of Leonardite was used as a source matrix of organic matter. An eluotropic series was assembled and sequential fractionation was performed by extraction on a Soxhlet apparatus. The original humic acid and fractions were characterized by elemental analysis (EA) and thermogravimetric analysis (TGA), followed by Fourier transform infrared spectrometry (FTIR), molecular absorption spectrometry (UV/VIS), fluorescence spectrometry and potentiometric titration. Atomic ratios were determined from the results of the elemental analysis. From the measured UV/Vis and fluorescence excitation and emission spectra, the absorption coefficients, resp. fluorescence coefficients. Used fractionation method proved to be a suitable method for studying HA structure. A total of 62 wt. % of initial materiál was extracted, indiivdual fraction amounted from 0.36–30.92 wt. %. From the results of the structural analysis, it is clear that with increasing polarity of the organic solvent, fractions with long aliphatic chains were first isolated and their aromaticity graddualy increased. Non-polar organic solvents were suitable for the extraction of lipid-like coumpounds, while the most polar organic fractions were rich in polar groups and their structual parameters were close to the original humic acid. The fraction extracted with acetonitrile was the most unique fraction. This fraction was rich on nitrogen and amine groups and was similar to protein-like structures. In the last two fractions, extracted with alcohols, a significant bathochromic shift typical of fluorophore type V was observed. Among other things, they were also characterized by a higher content of plant carbohydrate residues.
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Influence of the Application of Lignite on the Distribution of Organic Carbon in Soil
Širůček, David ; Záhora, Jaroslav (referee) ; Kalina, Michal (advisor)
This diploma thesis is focused on optimization of sequential chemical fractionation method to humeomics in order to be useful for determination of organic matter content and distribution and also organic elements in soil. Subsequently, the optimized method is used to assess the efect of lignite application as soil support on these soil characteristics. For these purposes, there were three source matrices of organic matter (lignite, soil and annual soil extraction after lignite application) fractionated by sequential chemical analysis. In parallel, these samples were also fractionated by classic alkaline extraction to obtain the so-called extractable fraction of organic matter (NOM). Individual fractions from sequential chemical fractionation as well as NOM samples were characterized by methods of elemental analysis (determination of organic elements), thermogravimetry (contents of ash, organic matter and moisture) and FTIR spektrometry (structural analysis). The results obtained from a large range of data from all humeomics fractions and NOM fractions showed that the method of sequential chemical fractionation gives higher yields of organic matter compared to classic alkaline extraction. Another indisputable advantage is the fact that the obtained fractions divided according to solubility and strenght of binding to soil inorganics can be better characterized by physical-chemical methods, which provides more detailed information about soil organic matter. The results of the work also show that in order for lignite as a support substance to significantly affect soil properties, a longer time, multiple sampling and repetition of individual fractionations would be needed.
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Sequential fractionation of organic matter of humic acid isolated from Leonardite
Krist, Tomáš ; Doskočil, Leoš (referee) ; Enev, Vojtěch (advisor)
The aim of the diploma thesis was to optimize the sequential fractionation method of organic matter to be used for physico-chemical characterization of extracted fractions. Humic acid isolated from oxidized brown coal of Leonardite was used as a source matrix of organic matter. An eluotropic series was assembled and sequential fractionation was performed by extraction on a Soxhlet apparatus. The original humic acid and fractions were characterized by elemental analysis (EA) and thermogravimetric analysis (TGA), followed by Fourier transform infrared spectrometry (FTIR), molecular absorption spectrometry (UV/VIS), fluorescence spectrometry and potentiometric titration. Atomic ratios were determined from the results of the elemental analysis. From the measured UV/Vis and fluorescence excitation and emission spectra, the absorption coefficients, resp. fluorescence coefficients. Used fractionation method proved to be a suitable method for studying HA structure. A total of 62 wt. % of initial materiál was extracted, indiivdual fraction amounted from 0.36–30.92 wt. %. From the results of the structural analysis, it is clear that with increasing polarity of the organic solvent, fractions with long aliphatic chains were first isolated and their aromaticity graddualy increased. Non-polar organic solvents were suitable for the extraction of lipid-like coumpounds, while the most polar organic fractions were rich in polar groups and their structual parameters were close to the original humic acid. The fraction extracted with acetonitrile was the most unique fraction. This fraction was rich on nitrogen and amine groups and was similar to protein-like structures. In the last two fractions, extracted with alcohols, a significant bathochromic shift typical of fluorophore type V was observed. Among other things, they were also characterized by a higher content of plant carbohydrate residues.
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Influence of the Application of Lignite on the Distribution of Organic Carbon in Soil
Širůček, David ; Záhora, Jaroslav (referee) ; Kalina, Michal (advisor)
This diploma thesis is focused on optimization of sequential chemical fractionation method to humeomics in order to be useful for determination of organic matter content and distribution and also organic elements in soil. Subsequently, the optimized method is used to assess the efect of lignite application as soil support on these soil characteristics. For these purposes, there were three source matrices of organic matter (lignite, soil and annual soil extraction after lignite application) fractionated by sequential chemical analysis. In parallel, these samples were also fractionated by classic alkaline extraction to obtain the so-called extractable fraction of organic matter (NOM). Individual fractions from sequential chemical fractionation as well as NOM samples were characterized by methods of elemental analysis (determination of organic elements), thermogravimetry (contents of ash, organic matter and moisture) and FTIR spektrometry (structural analysis). The results obtained from a large range of data from all humeomics fractions and NOM fractions showed that the method of sequential chemical fractionation gives higher yields of organic matter compared to classic alkaline extraction. Another indisputable advantage is the fact that the obtained fractions divided according to solubility and strenght of binding to soil inorganics can be better characterized by physical-chemical methods, which provides more detailed information about soil organic matter. The results of the work also show that in order for lignite as a support substance to significantly affect soil properties, a longer time, multiple sampling and repetition of individual fractionations would be needed.
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