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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Quantitative analysis of Scratch assay and Colony formation assay data using MATLAB
 Ingrová, Kateřina ; Chmelíková, Larisa ; Zumberg, Inna
Utilizing Scratch and Colony formation assays is crucial for understanding cell migration and proliferation dynamics. In this study, we present novel approaches utilizing MATLAB for the processing of images obtained from these assays. We demonstrate the effectiveness of our approaches through validation experiments on human osteosarcoma SaOS-2 cells, showcasing their applicability across different experimental conditions. Moreover, the inhibitory effect of two concentrations of gentamicin (0.1 mg/ml, 0.3 mg/ml) on the migration and clonogenicity of SaOS-2 cells was declared.
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Study of the effect of zinc oxide nanoparticles on cell migration
 Kutějová, Monika ; Zumberg, Inna (referee) ; Chmelíková, Larisa (advisor)
Zinc oxide nanoparticles (ZnO NPs) are widely used across various industries due to their properties, and with the development of nanomaterials, they are also finding applications in medicine. This bachelor’s thesis investigates the cytotoxic effects of these nanoparticles on cultured cell lines. The study focused on the human osteosarcoma cell line Saos-2 and the fibroblast cell line NIH-3T3. During in vitro experiments, the cells were exposed to different concentrations of ZnO NPs. The cytotoxicity of each concentration was assessed using the MTT assay, which provided information on cell viability. Based on the MTT assay results, appropriate concentrations of ZnO NPs were selected for conducting a Scratch assay, with the highest concentration corresponding to the determined inhibitory concentration for NIH-3T3. The cytotoxic effects were thus evaluated by studying cell migration. Subsequent statistical analysis determined the extent of cytotoxic effects of ZnO NPs depending on the concentration and cell type.
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