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Micropropagation of Satureja Montana L.
 Zunová, Tereza ; Fernández Cusimamani, Eloy (advisor) ; Michaela, Michaela (referee)
Winter savory (Satureja montana L.) is a plant occurring in the Mediterranean and the Black Sea region. For its high content of essential oils is widely used in culinary and traditional medicine. Savory is propagated generative and vegetative. Seeds germinate slowly, and therefore, the vegetative propagation is faster, especially in in vitro conditions may be propagated a large amount of plant material in a short time. The aim of this thesis was to investigate the growth effect of cytokinins on regeneration and growth plants from nodal segments of S. montana L. Nodal segments were cultured on six variants of medium supplemented with 0.5; 1 and 1.5 mg/l 6-benzylaminopurine (BAP) and 0.5; 1 and 1.5 mg/l kinetin (KIN). Variant without growth regulators served as a control. Culture conditions were 25/20 °C day/night, light intensity 3000 lx and daily period of 16/8 hours. After 6 weeks of measurements were evaluated parameters such as: number of shoots, plant height, the number of newly created nodes and the number and length of roots. In respect of the coefficient of micropropagation (i.e. the number of newly created nodes on the plant) was statistically significantly best variant medium supplemented with 1.5 mg/l KIN where on average create 8.88 +/- 2.87 nodes. At the height of the plant which was highest in the variant with a concentration 1.5 mg/l KIN (2.27 +/- 1.33) and the number of shoots with highest average value in a concentration 1 mg/l BAP (2.87 +/- 1.19) encore of growth regulators were not statistically proven. High concentrations of BAP and KIN inhibited the induction of roots and from variants transferred to ex vitro conditions were regenerated 46 % plants. Adding BAP and KIN into the medium has an influence on plant regeneration of Satureja montana L.
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