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Quality control of herbal drug Sambuci fructus fom cultivated plants.
Studená, Hana ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Bc. Hana Studená Supervisor: Doc. RNDr. Jiřina Spilková, CSc. Consultant: RNDr. Anna Polášková Title of diploma thesis: Quality control of herbal drug Sambuci fructus from cultivated plants The main aim of this thesis was to verify the quality of elderberry fruits of the cultivars Sambo, Sambu, Samdal, Samyl and of the wild elderberry according to tests specified in the Czech Pharmacopoeia 2009 and the Czech Pharmaceutical Codex 1. Loss on drying, total ash, ash insoluble in hydrochloric acid were determined. Another aim of this thesis was to compare certain cultivars of elderberry according to the content of anthocyanins in different parts of the fruits. A new modification of the spectrophotometric analysis using the pH-differential method was applied and its linearity and accuracy verified. Further, stability of the samples under given conditions was studied. A loss of anthocyanins content was observed, which was lower than 10 percent. The highest amount of anthocyanins was found in the cultivars Samyl and Samdal, the lowest amount in Sambu and wild elderberry. Although the determined quantities of anthocyanins differed in various parts of the fruit, the amount of anthocyanins in exocarp was...
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Quality control of herbal drug Sambuci fructus fom cultivated plants.
Studená, Hana ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Bc. Hana Studená Supervisor: Doc. RNDr. Jiřina Spilková, CSc. Consultant: RNDr. Anna Polášková Title of diploma thesis: Quality control of herbal drug Sambuci fructus from cultivated plants The main aim of this thesis was to verify the quality of elderberry fruits of the cultivars Sambo, Sambu, Samdal, Samyl and of the wild elderberry according to tests specified in the Czech Pharmacopoeia 2009 and the Czech Pharmaceutical Codex 1. Loss on drying, total ash, ash insoluble in hydrochloric acid were determined. Another aim of this thesis was to compare certain cultivars of elderberry according to the content of anthocyanins in different parts of the fruits. A new modification of the spectrophotometric analysis using the pH-differential method was applied and its linearity and accuracy verified. Further, stability of the samples under given conditions was studied. A loss of anthocyanins content was observed, which was lower than 10 percent. The highest amount of anthocyanins was found in the cultivars Samyl and Samdal, the lowest amount in Sambu and wild elderberry. Although the determined quantities of anthocyanins differed in various parts of the fruit, the amount of anthocyanins in exocarp was...
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Microscopic characterization of flowers from culivars of Sambucus nigra L.
Andělová, Veronika ; Spilková, Jiřina (advisor) ; Kašparová, Marie (referee)
Sambucus Nigra belongs to the most favourite plants of our national folk medicine. It has important place in pharmacy and food industry. It is know in our environment mainly in wild form however increasing interest and demand for qualitative balanced drug contributed to cultivation of cultural varieties in plantations. Flower of Sambucus Nigra contains mostly flavonoids. For receiving of the most quality drug is necessary to follow the rules of harvesting and drying. Currently is drug obtained only just from the plants in wild nature. The goal of thesis was valorising of all characteristic features of flowers Sambacus Nigra and differentiation between cultural varieties and wild forms with using microscopic test of identity stated in Czech Pharmacopoeia 2009. Characteristic flower features of Albida, Alleső, Aurea, Bohatka, Dana, Haschberg, Juicy, Kőrsőr, Madona, Pregarten, Riese aus Vobloch, Tulbing and wild form of Sambuca Nigra were examined. All samples were brightened in solution of chloral hydrate and afterwards microscopic preparations were created. Results were photographically documented. Among the observed features belongs: cells containing sand of calcium oxalate, stomata and epidermal cells of crown leaf. Besides were observed pollen grains, flowers venation or cuticle corrugation of...
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