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National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
Fluorescence of organic dye in free form or bound on metal nanoparticles analysis using confocal microscopy
 Mocko, Štefan ; Chmelíková, Larisa (referee) ; Čmiel, Vratislav (advisor)
This bachelor`s thesis deals with the long-term analysis of the fluorescence of rhodamine dye, which is linked to the ferric SPIO nanoparticles. The first part introduces the necessary scientific basis for understanding the physical phenomenon of fluorescence. It also focuses on the hardware and software for the development of long-term analysis of fluorescence for mesenchymal stem cells.Finally, there is described an analytical software to work with measured data, which was developed for this work.
Detailed record
Realisation of method for fluorescence lifetime and spectral changes evaluation using advanced confocal microscopy techniques
 Rúbal, Radek ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
Content is focused on fluorescence lifetime imaging techniques. Fluorescence lifetime is computed from data acquired with using of Leica TCS SP8X confocal microscope sequential scanning. Algorithms and software for the computation, imaging and analysis of fluorescence lifetime is presented. Software is allowing both 2D and 3D imaging of fluorescence lifetime. Techniques are used for fluorescence lifetime imaging of mesenchymal cells and fibroblasts tainted with SPIO-Rhodamin complex.
Detailed record
Fluorescence of organic dye in free form or bound on metal nanoparticles analysis using confocal microscopy
 Mocko, Štefan ; Chmelíková, Larisa (referee) ; Čmiel, Vratislav (advisor)
This bachelor`s thesis deals with the long-term analysis of the fluorescence of rhodamine dye, which is linked to the ferric SPIO nanoparticles. The first part introduces the necessary scientific basis for understanding the physical phenomenon of fluorescence. It also focuses on the hardware and software for the development of long-term analysis of fluorescence for mesenchymal stem cells.Finally, there is described an analytical software to work with measured data, which was developed for this work.
Detailed record
Realisation of method for fluorescence lifetime and spectral changes evaluation using advanced confocal microscopy techniques
 Rúbal, Radek ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
Content is focused on fluorescence lifetime imaging techniques. Fluorescence lifetime is computed from data acquired with using of Leica TCS SP8X confocal microscope sequential scanning. Algorithms and software for the computation, imaging and analysis of fluorescence lifetime is presented. Software is allowing both 2D and 3D imaging of fluorescence lifetime. Techniques are used for fluorescence lifetime imaging of mesenchymal cells and fibroblasts tainted with SPIO-Rhodamin complex.
Detailed record

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