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Citlivost plůdku hlavatky obecné (Hucho hucho) k anestetikům Eugenol, MS-222 a 2-fenoxyetanol.
KAREŠ, Martin
This diploma thesis, in the introduction, summarizes the known information about anesthesia with the physiological effect of anesthetics on fish, including a description of the various stages of anesthesia. The following are the detailed characteristics of the anesthetics used (Eugenol, MS-222, 2-phenoxyethanol and Propiscin) and describes the biology and breeding of the studied fish species - Hucho hucho and related species. The practical part of the work deals with the assessment of the influence of individual anesthetics (Eugenol, MS-222, 2-phenoxyethanol and Propiscin) on the onset of individual phases of anesthesia (2, 3a, 3b, 4) and the disappearance phase (3b, 3a, 2,). Unless otherwise stated, the average piece weight of the experimental subjects used was in the range. In the case of the anesthetic Eugenol, the effect of temperature (in the range of 5-15°C) and the concentration of the anesthetic bath (in the range of 0.02-0.04 ml.l-1) and the effect of different fish sizes (in the case of 3 groups in the range of average piece weight 4-353g, at a temperature of 10°C and an anesthetic concentration of 0.03 ml.l-1). The efficacy of the anesthetics MS-222, 2-phenoxyethanol and Propiscin was monitored only at 10°C, each at 5 different concentrations (MS-222 at a concentration of 60-140 mg.l-1,2-phenoxyethanol at a concentration of 0.40-0.60 ml.l-1 and Propiscin at a concentration of 0.5-1.5 ml.l-1). Prior to the actual experiments, the fish were adapted for several days to the temperatures at which the monitoring was performed, the day before the actual experiments the fish were not fed. The duration of the experimental fish's stay in the anesthetic bath was always 10 minutes, after which the fish were immediately transferred to a container of clean water of identical temperature and their observation was continued. The achieved results of the onset of individual phases of anesthesia and its fading in individual variants of the experiment are characterized by the average length of time in seconds and the standard deviation. In most cases, the highest onset times of anesthesia were 5°C and 7.5°C. The lowest values of the onset of anesthesia were 12.5°C. The average values of anesthesia fading decreased with higher temperature in most cases, but with higher concentration the onset of fading phases prolonged. The onset of the individual phases of anesthesia and fading were influenced by concentration, temperature, and weight. Orientation test of anesthetics MS-222, 2-phenoxyethanol and Propiscin at 10°C. The mentioned anesthetics showed the same trend, a gradual decrease in the onset of the anesthesia phases with increasing concentration. In the case of MS-222, 20% mortality was monitored at a concentration of 140 mg.l-1. Propiscin shows a significantly longer time for anesthesia to subside at a concentration of 1 ml.l-1. In the monitored size categories at a temperature of 10°C and a concentration of 0.03 ml.l-1 Eugenol, there was no clear trend of the dependence of the onset of individual phases of anesthesia on the size of fish.
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Effects of anaesthetics on barbel
ŘEŽÁBEK, Josef
The aim of this study was to assess the effects of the four most used anaesthetics in European aquaculture MS 222, clove oil, 2-phenoxyethanol and Propiscin on barbel (Barbus barbus). The effects of anaesthetics were assessed based on haematological profile, biomarker of oxidative stress and antioxidant enzymes. This study contributed to the expansion of knowledge on the safety of tested anaesthetics and selected safe anaesthetics for barbel. Barbels were exposed to a 10-minute anaesthesia with MS 222 (in recommended concentration 100 mg×l-1), clove oil (in recommended concentration 33 mg×l-1), 2-phenoxyethanol (in recommended concentration 0.4 ml×l-1) and Propiscin (in recommended concentration 1 ml×l-1). The effects of anaesthetics were evaluated immediately after 10 min. anaesthesia and 24 hours after anaesthesia. Anaesthesia with MS 222, clove oil, 2-phenoxyethanol and Propiscin had no significant effect on haematological indices, level of oxidative stress (TBARS) and activity of glutathione reductase in barbel tissues. The activity of catalase was significantly increased in the muscle 24 h after anaesthesia of all anaesthetics compared to the control. Superoxide dismutase activity was changes in all experimental groups (immediately after 10 min. anaesthesia and 24 hours after anaesthesia). The tested anaesthetics not altered hematopoietic tissue and had not effect on the level of lipid peroxidation in barbel´s tissues. The results of this study suggest that the antioxidant systems of barbel are altered by Propiscin anaesthesia, but they are slightly affected by MS 222, clove oil, and 2-phenoxyethanol anaesthesia. On the basis of the results of this thesis, for anaesthesia of barbel we can recommend clove oil and 2-phenoxyethanol as an alternative MS 222.
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Comparison of the effectiveness and efficiency of the four common anesthetic used in fisheries for burbot (Lota lota L.)
RŮŽEK, Martin
The aim of this study was to compare the effect of four anaesthetics on haematological and biochemical blood profiles in burbot (Lota lota L.). Both blood profiles of burbot were evaluated 10 min and 24 h after anaesthesia with MS-222 (100 mg.L-1), clove oil (33 mg.L-1), 2-phenoxyethanol (0.30 ml.L-1), Propiscin (1 ml.L-1) and were compared to non-anaesthetised control. The tested anaesthetics did not have any effect on haematological profile of burbot. The exposure to clove oil, 2-phenoxyethanol and Propiscin significantly influenced level of ammonia and glucose. The level of lactate was significantly increased following anaesthesia with 2-phenoxyethanol and Propiscin. The levels of total protein, aspartate aminotransferase and calcium were higher with clove oil, 2-phenoxyethanol and Propiscin compare to control. The use of MS-222 showed the smallest effect on haematological and biochemical blood profile and is recommended as a suitable anaesthetic for burbot.
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Effect of anaestetics on fish - review
MALÝ, Jan
Bachelor thesis "Effect of anaestetics on fish" has been written as a review. The aim was to elaborate and create the review about the effect of anaestetics on fish. There can be found effects of 12 widely used anaestecics in the European and world aquacultural regions. I have focused mainly on the four mostly used anaestetics in the Czech aquaculture, especially on the clove oil, MS-222, 2-phenoxyethanol and propiscin. To work properly out this bachelor thesis, I have used home and foreign sources found in the scientis database. My bachelor´s thesis is bringing a complete summary concerning the effect of anaestetics on fish and that is why it can be used for similar surveys (purpose) in the field of fish anaesthesia, other use of anaestecics in aquaculture and fish health protection in accordance with the Animal protection law Nr. 359/2012, which has replaced the former law Nr. 246/1992.
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