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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Biotechnological production of PHA employing selected extremophiles
 Kurzová, Pavlína ; Müllerová, Lucie (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on study of production of polyhydroxyalkanoates (PHA) by bacteria Halomonas salina (CCM 4361T). Theoretical part deals with the properties and biosynthesis of PHA and chracterization of extremophiles bacteria. The experimental part is focused on cultivation of bacteria on different substrates and concentration of sodium chloride in medium for optimal production of PHA. Content of biomass and PHA were analyzed by spektrofotometry and gass chromatography with FID. The fructose proved to be the most suitable substrate for a production of PHA, with a content of 12,55 % of PHA in biomass. Optimal concetration of NaCl was found at 80 g/l. Bacteria were investigated by molecular technique polymerase chain reaction (PCR) and detected by gel elctrophoresis. Test confrimed opresence of phaC gene encoding for PHA synthase, enzyme which is necessary for PHA biosynthesis.
Detailed record
Biotechnological production of PHA employing selected extremophiles
 Kurzová, Pavlína ; Müllerová, Lucie (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on study of production of polyhydroxyalkanoates (PHA) by bacteria Halomonas salina (CCM 4361T). Theoretical part deals with the properties and biosynthesis of PHA and chracterization of extremophiles bacteria. The experimental part is focused on cultivation of bacteria on different substrates and concentration of sodium chloride in medium for optimal production of PHA. Content of biomass and PHA were analyzed by spektrofotometry and gass chromatography with FID. The fructose proved to be the most suitable substrate for a production of PHA, with a content of 12,55 % of PHA in biomass. Optimal concetration of NaCl was found at 80 g/l. Bacteria were investigated by molecular technique polymerase chain reaction (PCR) and detected by gel elctrophoresis. Test confrimed opresence of phaC gene encoding for PHA synthase, enzyme which is necessary for PHA biosynthesis.
Detailed record

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