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Výskyt a diverzita amphistomních motolic u skotu
VOGALOVÁ, Pavlína
The thesis deals with gastric woodlice of the family Paramphistomidae, which are common parasites of ruminants. Their immature stages can cause severe pathological changes in the host's digestive tract, and massive infection can lead to host death. Adult parasites cause infections of the rumen mucosa, resulting in lower production. The prevalence and diversity of these flukes is currently not systematically monitored. The aim of this study is to describe the occurrence and diversity of gastric flukes in cattle in the Czech Republic and Austria. The practical part consists of examination of dairy cattle faeces from different herds for the presence of eggs using standardised parasitological methods, optimization of a PCR method for direct detection of flukes from the faeces, genotyping of the flukes found, processing of the results using appropriate statistical methods, and comparison with published data.
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Detection of persistent forms of Borrelia burgdorferi sensu stricto in infected mice after antibiotic treatment
RAMZY, Ida
Antibiotic treatment of Lyme disease is a challenging procedure with unexpected outcomes that may arise sometime. According to standard protocols recommended by the Centers for Disease Control and Prevention, USA (CDC) for early Lyme disease, a short course of oral antibiotics cures the majority of cases. This thesis deals with the analysis of the efficiency of 2 majorly used antibiotics, doxycycline and amoxicillin, in elimination of Borrelia infection on laboratory mice model after 2 weeks of treatment. Our results confirmed the presence of persistent forms of spirochetes in mice tissues after antibiotic treatment using the spirochete cultivation method in liquid MKP medium.
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Diagnosis of leishmaniases in humans and dogs
Černá, Eva ; Spitzová, Tatiana (advisor) ; Votýpka, Jan (referee)
Leishmaniasis is a serious disease caused by parasites that affects both people and animals. In people, this disease has three forms, cutaneous, mucocutaneous and visceral form. Visceral form is lethal if it's left untreated. Leismaniasis is usually diagnosed by using regular parasitological methods based on histocytological analysis. In the last few years, there has been a considerable progress in serological and molecular diagnostics. This thesis focuses on description and comparison of the traditional and the new diagnostic methods. Attention is paid primarily to the new methods, therefore serological and molecular. These methods are compared by their sensitivity, specifity, field application potential, financial costs and time consumption. Both human and canine diagnostics are mentioned due to the fact that dogs are a significant reservoir. Key words leishmaniasis, diagnostics, parasitology, humans, dogs, method, molecular, serological, sensitivity, specifity
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