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Content of rutin in selected elderberry cultivars
Píšťková, Magdalena ; Hrstka, Miroslav (referee) ; Vespalcová, Milena (advisor)
The aim of this diploma thesis was to determine the rutin content in springs and leaves of 19 elderberry species (Sambucus nigra L.) with use of liquid chromatography. Furthermore the rutin content was determined in its significant sources – rue and buckwheat. The observed values were compared in order to answer the question whether it is useful to use the elderberry spring and leaves as a new source of rutin. Theoretical part of the thesis describes elderberry in general, its chemical composition and its usage in medicine. Following part concentrates on important substances contained in elderberry – flavonoids. Also principles of extraction techniques and HPLC are discussed. In practice the rutin was gained from plant matrix with use of pressurized hot water extraction (PHWE) which was performed in three five minutes long cycles. Conditions during the PHWE were set up to 80 °C and 150 bars. Obtained extract was purified with use of SPE columns and analyzed by HPLC with DAD detector (wave length – 360 nm). HPLC was performed with SUPELCOSILTMLC-8DB column. The mobile phase was composed of MeOH, H2O, and HCOOH in ration of 36:61,5:2,5. The set up flow of mobile phase was 1 ml/min. In the leaves the highest rutin content was determined in Pregarten – 26,34 ± 0,50 mg/g. On the other hand the lowest rutin content in leaves was determined in Körsör – 3,74 ± 0,13 mg/g. In springs the obtained results were as follow. The highest content was determined in Samyl – 4,87 ± 0,01 mg/g and the lowest content was determined in Heidegg 13 springs – 0,56 ± 0,01 mg/g. Also the HPLC detection limit – 6,3 . 10-5 mg/ml and quantitation limit – 2,1 . 10-4 mg/ml were determined for rutin.
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Financing of Companies Through Entry into the Alternative Market PSE
Štěpánek, Marek ; Ptáček, Roman (referee) ; Meluzín, Tomáš (advisor)
Master‘s thesis is focused on the possibility of financing a company through entering the alternative market of the Prague Stock Exchange Ltd. The alternative market of the Prague Stock Exchange is the START Market, which is suitable for smaller and innovative czech companies. This is about an unregulated market, which means that the conditions of admission conditions and trading rules are set by the stock exchange. The thesis defines the basic starting points, which are connected with the capital market, the market organizer and the initial public offering of stocks (IPO).
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Determination of organic compounds in biochar produced by microwave torrefaction of biomass
Meindl, Jiří ; Vávrová, Milada (referee) ; Mravcová, Ludmila (advisor)
The thesis is focused on a determination of polycyclic aromatic hydrocarbons (PAHs) contained in dried pelletized sewage sludge and pelletized biochar. Biochars were made in mild conditions by microwave torrefaction of prepared sewage sludge. There were analyzed and quantified the 34 of standardized PAHs compounds in two series. The first serie, also called “Sada 1”, has been aimed at comparison of extraction methods for the chosen sample of sewage sludge and the sample of biochar. In serie “Sada 1”, there were compared efficiencies of chosen type of solvent or solvent mixture by comparison of yields for 34 standardized analytes in a sample of biochar and a sample of sewage sludge. There were compared also to total yields of PAHs and to number of quantified compounds in analyzed samples. The most reliable extraction method has been used for the next analyses of samples in the second serie called “Sada 2”. In Sada 2, there were compared different samples of the same type (e.g. biochar, sludge). The origin of sewage sludge (small or big sewage treatment plant expressed as PE) and used additives (cellulose, chaff, hay) as modificators for torrefaction process were variables for different type of sample. The results of analysis were identification of the most suitable sewage sludge and additive to be used as modificator for microwave torrefaction process. The main goal of correctly chosen sludge and additive was to minimize production of PAH’s during torrefaction and in samples of biochar.
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Extraction of selected elderberry flavonoids for food industry purposes
Grulichová, Hana ; Hrstka, Miroslav (referee) ; Vespalcová, Milena (advisor)
Amount of rutin and quercetin in bark and leaves from different type of Sambucus nigra was determined in this diploma thesis. Records of amounts were compared with amounts in significant sources of these flavonoids. Matters from raw matrix were obtained with pressurized solvent extraction (PSE) and pressurized hot water extraction (PHWE). There was use temperature 120°C, pressure 150 bar, three times 5-min cycles for PSE. There were used 80°C, 150 bar, three 5-min cycles for rutin extracted with PHWE and the same condition were used for extraction of quercetin only the temperature was changed to 100 °C. Analysis of rutin and quercetin were carry out high performance liquid chromatography (HPLC) with diode array detection (DAD), where was used SUPELCOSILTM LC 8DB column (5µm; 250 x 4.6 mm). Methanol: water: formic acid (36: 61.5: 2.5, pH 2.17 2.28) was used like a mobile phase. The flow of the mobile phase was set up 0.7 ml/min. Size of sample was 10 µl. The most amount of rutin was determined in the leaves of wild kind of Sambucus nigra with methanol extraction and hot water too. Extraction with PHWE was more effectively. There were determined 5.58 mg of rutin in one gram of sample from wild Sambucus nigra. There were determined the smallest amount of rutin in Sambucus nigra Körsör. There was found only 0.13 mg/g with extraction PHWE. The quercetin wasn’t determined in any Sambucus nigra leaves. There were found both of the flavonoids (rutin and quercetin) in the branch of Sambucus nigra. The rutin was determined in the small amount in wild kind but it was found in the important amount in Sambucus nigra cultivar. Whereas the quercetin was found in higher concentration than rutin when was finding the ideal conditions for extraction of branch. The quercetin was chosen like more important in light of amount. In the end the quercetin wasn’t detected in any Sambucus nigra cultivars. The most amount of quercetin was determined in wild sambucus nigra, 0.24 mg/g. The most amount of rutin was found in Sambucus nigra Albida, 2.33 mg/g.
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Power over Ethernet
Hykš, Matěj ; Šilhavý, Pavel (referee) ; Koton, Jaroslav (advisor)
The bachelor thesis focuses on the topic of powering network elements using Power over Ethernet shortly PoE. The work focuses more in detail on the functions of the 802.3af and 802.3at standards, which are described in detail and explains their functionality on specific examples of PD chips. Then it summarizes the measurement methodology used for 3 devices belonging to the PSE category and 4 devices from the PD category. In both cases, it focuses on their analog and digital communication.
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Vlivy působící na celkovou jakost výsekového masa a odchylky jakosti
Kolísková, Adéla
The term meat is defined in the bachelor's thesis, and its consumption in recent years is also listed here. I also deal with the chemical composition and explanation of the concept of quality, which is the result of nine quality characteristics. Furthermore, the factors that influence the final quality of the meat are described here. Factors can be divided into intravital and postmortem. Postmortem processes and slaughtering processing, which also contribute to the final quality of the products, are described in more detail. The following is a list and description of some forms of meat spoilage. The work also characterizes the most frequent quality deviations, supplemented by the causes of their occurrence and the possibility of their identification.
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Power over Ethernet
Hykš, Matěj ; Šilhavý, Pavel (referee) ; Koton, Jaroslav (advisor)
The bachelor thesis focuses on the topic of powering network elements using Power over Ethernet shortly PoE. The work focuses more in detail on the functions of the 802.3af and 802.3at standards, which are described in detail and explains their functionality on specific examples of PD chips. Then it summarizes the measurement methodology used for 3 devices belonging to the PSE category and 4 devices from the PD category. In both cases, it focuses on their analog and digital communication.
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PSE as One of the Possibilities of Primary Prevention
Říhová, Ivana ; Valenta, Josef (advisor) ; Švandová, Martina (referee)
This thesis is focused on the possibility of using Personal and Social Education in implementing effective primary prevention program in elementary schools. After the theoretical analysis of primary prevention and Personal and Social Education, the second part of the thesis presents and analyzes a proposed model of prolonged non-specific primary prevention, which is based mainly on the activities of school prevention methodologist. The methodologist is educated in social pedagogy and professionally trained in Personal and Social Education. The school prevention methodologist is employed full-time for this position, he does not teach any other subject but Personal and Social Education. In this part of the thesis there are discussed demands on school prevention methodologist activities and difficulties of presented primary prevention model. The last chapter presents supporting research that took place at four elementary schools in Prague. Observations of the teaching Personal and Social Education, conversations with its teacher and also an analysis of the Minimum Preventive Program of the school were made there.
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Financing of Companies Through Entry into the Alternative Market PSE
Štěpánek, Marek ; Ptáček, Roman (referee) ; Meluzín, Tomáš (advisor)
Master‘s thesis is focused on the possibility of financing a company through entering the alternative market of the Prague Stock Exchange Ltd. The alternative market of the Prague Stock Exchange is the START Market, which is suitable for smaller and innovative czech companies. This is about an unregulated market, which means that the conditions of admission conditions and trading rules are set by the stock exchange. The thesis defines the basic starting points, which are connected with the capital market, the market organizer and the initial public offering of stocks (IPO).
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Determination of organic compounds in biochar produced by microwave torrefaction of biomass
Meindl, Jiří ; Vávrová, Milada (referee) ; Mravcová, Ludmila (advisor)
The thesis is focused on a determination of polycyclic aromatic hydrocarbons (PAHs) contained in dried pelletized sewage sludge and pelletized biochar. Biochars were made in mild conditions by microwave torrefaction of prepared sewage sludge. There were analyzed and quantified the 34 of standardized PAHs compounds in two series. The first serie, also called “Sada 1”, has been aimed at comparison of extraction methods for the chosen sample of sewage sludge and the sample of biochar. In serie “Sada 1”, there were compared efficiencies of chosen type of solvent or solvent mixture by comparison of yields for 34 standardized analytes in a sample of biochar and a sample of sewage sludge. There were compared also to total yields of PAHs and to number of quantified compounds in analyzed samples. The most reliable extraction method has been used for the next analyses of samples in the second serie called “Sada 2”. In Sada 2, there were compared different samples of the same type (e.g. biochar, sludge). The origin of sewage sludge (small or big sewage treatment plant expressed as PE) and used additives (cellulose, chaff, hay) as modificators for torrefaction process were variables for different type of sample. The results of analysis were identification of the most suitable sewage sludge and additive to be used as modificator for microwave torrefaction process. The main goal of correctly chosen sludge and additive was to minimize production of PAH’s during torrefaction and in samples of biochar.
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