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Medium voltage substation automation using control system COM600 and standard IEC61850
Havelka, Tomáš ; Štaffa, Jan (referee) ; Háze, Jiří (advisor)
This work deals with problematic of standard IEC61850, mainly with chapter IEC 61850-9-2 LE which is commonly known as Process bus communication. There are described advanced tools of the control system COM600 with detail description of their configuration in this work. It also deals with configuration of IEC 61850-9-2 LE with Relion family protection relays and its implementation to the control system COM600. The main aim of this work is focused on configuration of IEC 61850-9-2 standard with protection relays, configuration and detail description of COM600 advanced tools, description of Logic processor, further custom design and appliaction configuration global reset of Logic procesor in control system COM600.
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Large Extracellular Vesicles in Cell Culture and Blood: Role in Prion Transmission and Detection by Flow Cytometry
Soukup, Jakub
Prions (PrP) are the main cause of neurodegenerative diseases such as Scrapie in sheep, bovine spongiform encephalopathy, chronic wasting disease in deer, and Creutzfeldt-Jakob disease in humans. Although the cellular PrP (PrPC ) is involved in many cellular processes, its precise function still needs to be discovered. The disease is caused by the accumulation of a pathological form of PrP (PrPTSE ), which is caused by direct contact of PrPTSE and PrPC . PrP is anchored in the membrane by GPI and can be transmitted by cell-to-cell contact, tunnelling nanotubes, or extracellular vesicles (EVs). EV factions are divided by different biogenesis into exosomes, microvesicles, and apoptotic bodies. PrPTSE was found in exosomes and microvesicles, but these fractions were never compared to each other. The first aim of the doctoral thesis is a comparison of PrP content, prion-converting activity and infectivity in these fractions on CAD5 and N2a-PK1 cellular models of infection. We isolated a fraction of large EVs (20,000× g) and small EVs (110,000× g) by centrifugation from a conditioned medium. We characterised EVs by cryo-electron microscopy and western blot with Alix, TSG-101, CD63, CD9, and HSP70 markers. The contamination from other cellular compartments was checked by calnexin. EV fractions differed...
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Large Extracellular Vesicles in Cell Culture and Blood: Role in Prion Transmission and Detection by Flow Cytometry
Soukup, Jakub ; Holada, Karel (advisor) ; Šebestová Janoušková, Olga (referee) ; Živný, Jan (referee)
Prions (PrP) are the main cause of neurodegenerative diseases such as Scrapie in sheep, bovine spongiform encephalopathy, chronic wasting disease in deer, and Creutzfeldt-Jakob disease in humans. Although the cellular PrP (PrPC ) is involved in many cellular processes, its precise function still needs to be discovered. The disease is caused by the accumulation of a pathological form of PrP (PrPTSE ), which is caused by direct contact of PrPTSE and PrPC . PrP is anchored in the membrane by GPI and can be transmitted by cell-to-cell contact, tunnelling nanotubes, or extracellular vesicles (EVs). EV factions are divided by different biogenesis into exosomes, microvesicles, and apoptotic bodies. PrPTSE was found in exosomes and microvesicles, but these fractions were never compared to each other. The first aim of the doctoral thesis is a comparison of PrP content, prion-converting activity and infectivity in these fractions on CAD5 and N2a-PK1 cellular models of infection. We isolated a fraction of large EVs (20,000× g) and small EVs (110,000× g) by centrifugation from a conditioned medium. We characterised EVs by cryo-electron microscopy and western blot with Alix, TSG-101, CD63, CD9, and HSP70 markers. The contamination from other cellular compartments was checked by calnexin. EV fractions differed...
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Medium voltage substation automation using control system COM600 and standard IEC61850
Havelka, Tomáš ; Štaffa, Jan (referee) ; Háze, Jiří (advisor)
This work deals with problematic of standard IEC61850, mainly with chapter IEC 61850-9-2 LE which is commonly known as Process bus communication. There are described advanced tools of the control system COM600 with detail description of their configuration in this work. It also deals with configuration of IEC 61850-9-2 LE with Relion family protection relays and its implementation to the control system COM600. The main aim of this work is focused on configuration of IEC 61850-9-2 standard with protection relays, configuration and detail description of COM600 advanced tools, description of Logic processor, further custom design and appliaction configuration global reset of Logic procesor in control system COM600.
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