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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Characterization of liposomes as precursors for the preparation of models of cellular membrane using scattering techniques
 Gjevik, Alžběta ; Kalina, Michal (referee) ; Mravec, Filip (advisor)
The bachelor thesis presents a cellular membrane design based on lecithin, cholesterol and POPG basis. It summarizes formation mechanisms, optimization techniques and characterization methods of model cellular membranes. It focuses on preparation of liposomes with various lipid compositions as precursors for model membranes preparation and characterization. Small unilamellar liposomes were formed by thin layer evaporation, thin layer rehydration in phosphate buffer and sonication. Size and stability (-potential) of formed liposomes were measured using dynamic light scattering. Successful supported lipid bilayer formation on glass surface by vesicular fusion was tested using the most stable lipid composition. SLB was characterized by Z-scan fluorescence correlation spectroscopy.
Detailed record
Characterization of liposomes as precursors for the preparation of models of cellular membrane using scattering techniques
 Gjevik, Alžběta ; Kalina, Michal (referee) ; Mravec, Filip (advisor)
The bachelor thesis presents a cellular membrane design based on lecithin, cholesterol and POPG basis. It summarizes formation mechanisms, optimization techniques and characterization methods of model cellular membranes. It focuses on preparation of liposomes with various lipid compositions as precursors for model membranes preparation and characterization. Small unilamellar liposomes were formed by thin layer evaporation, thin layer rehydration in phosphate buffer and sonication. Size and stability (-potential) of formed liposomes were measured using dynamic light scattering. Successful supported lipid bilayer formation on glass surface by vesicular fusion was tested using the most stable lipid composition. SLB was characterized by Z-scan fluorescence correlation spectroscopy.
Detailed record

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