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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Preparation of HEK293 cell line expressing auxin transporter PIN7 and testing of inhibitors of auxin transport
 Petermannová, Romana ; Vaněk, Ondřej (advisor) ; Moserová, Michaela (referee)
Auxin is one of the most important plant hormones, which provides development of a plant. PIN1 and PIN7 proteins belong to the PIN family of transporters which is among the most important auxin efflux carriers. This thesis deals with the of AtPIN1 and AtPIN7 auxin efflux carriers (from Arabidopsis thaliana) in human embryonic kidney 293 cell line. Biological activity of these proteins was tested by using radiolabeled auxins accumulation. Further inhibitors of auxin transport have been tested - NPA, CHPAA and BFA.
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