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miRNA-mediated translational repression
Kučerová, Michaela ; Mašek, Tomáš (advisor) ; Beznosková, Petra (referee)
Recent models of miRNA-mediated translational repression favor initiation as the phase of translation, where the repression is primarily evoked. miRNA-induced silencing complex utilizes miRNA as a guide molecule to find its target mRNA. To inhibit mRNA translation and induce its decay, many other proteins are then recruited, e.g. GW182 protein, PAN2-PAN3 a CCR4-NOT deadenylation complexes, decapping enzyme and eIF4E-binding proteins. miRNA-mediated translational repression is then established by either their concerted action or their consecutive and coordinated binding to target mRNA. To date, several mechanisms have been described: GW182- mediated PABP displacement; GW182-independent translational repression; miRNA-mediated eIF4A1 dissociation or eIF4A2 recruitment; translational repression mediated by deadenylases CAF1 and CCR4; and 4E-T, eIF4E2, GIGYF2 translational repressor proteins recruitment. Here, I provide up-to-the-minute summarization of these mechanisms with the aim to extract any universality from them.
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