National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The use of genetic variability for the purposes of conservation biology: case studies of populations in captivity and nature
Opelka Somerová, Barbora ; Frynta, Daniel (advisor) ; Veselý, Milan (referee) ; Vojar, Jiří (referee)
2 Abstract In this dissertation thesis, I focused mainly on the topic of endangered species conservation in three projects, which were realized in cooperation with Prague ZOO. The other two subprojects, which are part of this dissertation, deal with the topic of genetic diversity analysis in nature and were realized in cooperation with institutions in Indonesia and Armenia. In all these projects I used a similar laboratory and computational methodology, although each project addressed different problems. In the main and probably the most important project, I studied the skinks of genus Tiliqua living in the Moluccas, New Guinea (Irian Jaya) and adjacent islands. I analyzed mitochondrial and nuclear markers to reveal the phylogenetic and population relationships. In the second project, I focused on the study of ex situ population of Mauremys annamensis in European captive breeding. The goal was to determine the haplotype diversity and detect the F1 hybrids in population. In the third project, I examined the genetic variability of Orlitia borneensis in European zoological gardens for the purposes of ex situ breeding program organization. The fourth project is focused on examination of genetic variability of Chilabothrus angulifer population, which is kept in European breeding. I studied ...
Genetic variability in a population of endangered turtle Orlitia borneensis kept in European zoos
Somerová, Barbora ; Frynta, Daniel (advisor) ; Hulva, Pavel (referee)
Malaysian Giant Turtle (Orlitia borneensis) in poorly known turtle with rapidly descending numbers in nature. Most animals of this species kept in European zoos and included in captive breeding program are confiscates of illegal trade and their locality of origin and taxonomic status are unknown. This study was aimed to assess genetic variation in founders of this population. We sequenced genes for the mitochondrial cytochrome b and found 23 haplotypes. The maximum sequence divergence was less than 1.5% and the phylogenetic structure of the haplotypes was only poorly supported. A close genetic similarity among sampled turtles was further confirmed by a sequencing of the nuclear R35 gene. Thus, the examined population of O. borneensis may be further treated as a single conservation unit.

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