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Correlation analysis of optically trapped particle motion
Koudelníček, Karel ; Jákl,, Petr (referee) ; Harabiš, Vratislav (advisor)
This bachelor’s thesis deals with the description of random (Brownian) motion of microscopic particle trapped by optical trap. This thesis provides overview of possible usage in biology. The major part of the thesis is dedicated to description of methods that allow characterize optical trap with etc. stiffness thanks to the random motion. First, the estimation methods are tested on the simulated data. Before using these methods for real data, it was necessary to modify them, specifically to eliminate crosstalk and frequency noise. The differences in the estimation of the stiffness from the autocorrelation function and the power spectrum were described in the conclusion.
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Optical tweezers for coherence-controlled holographic microscope
Straka, Branislav ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
In the master's thesis, there has been described and explained the principle of operation of the second generation coherence controlled holographic microscope (CCHM2) designed at the Brno University of Technology. There has also been listed theoretical description of the operation of the optical trap, together with the calculation of the forces acting on it, ways of measuring the stiffness of the optical trap and the principle of~creating a time-shared optical traps. The optical tweezers forming a separate module connectable to CCHM2 was designed. Simulation and optimization of parameters of the optical system, mechanical design, manufacturing documentation, current source to power the laser diode which allows to control the diode output power by the controller card connected to the PC was designed. The galvano-optics mirror angle is controlled by the PC card too. The optical tweezer has been designed, manufactured and tested in conjunction with the CCHM2.
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Components for laser micromanipulation in a coherence-controlled digital holographic microscope
Straka, Branislav ; Antoš, Martin (referee) ; Dostál, Zbyněk (advisor)
In the Bachelor's thesis, there was described the general principle of operation of the optical trap, along with various options for creating optical tweezers. The function and the main parameters of holographic microscope with the off-axis achromatic and spatially invariant interferometer were described, too. Two variations of laser tweezers connectable to coherence-controlled second generation holographic microscope were described in detail, calculated and designed at IPE VUT. The optimization of the two variations was performed in the software ZEMAX. A 3D model in the Autodesk Inventor PROFESSIONAL 2010 was created for the chosen design.
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Correlation analysis of optically trapped particle motion
Koudelníček, Karel ; Jákl,, Petr (referee) ; Harabiš, Vratislav (advisor)
This bachelor’s thesis deals with the description of random (Brownian) motion of microscopic particle trapped by optical trap. This thesis provides overview of possible usage in biology. The major part of the thesis is dedicated to description of methods that allow characterize optical trap with etc. stiffness thanks to the random motion. First, the estimation methods are tested on the simulated data. Before using these methods for real data, it was necessary to modify them, specifically to eliminate crosstalk and frequency noise. The differences in the estimation of the stiffness from the autocorrelation function and the power spectrum were described in the conclusion.
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|
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Optical tweezers for coherence-controlled holographic microscope
Straka, Branislav ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
In the master's thesis, there has been described and explained the principle of operation of the second generation coherence controlled holographic microscope (CCHM2) designed at the Brno University of Technology. There has also been listed theoretical description of the operation of the optical trap, together with the calculation of the forces acting on it, ways of measuring the stiffness of the optical trap and the principle of~creating a time-shared optical traps. The optical tweezers forming a separate module connectable to CCHM2 was designed. Simulation and optimization of parameters of the optical system, mechanical design, manufacturing documentation, current source to power the laser diode which allows to control the diode output power by the controller card connected to the PC was designed. The galvano-optics mirror angle is controlled by the PC card too. The optical tweezer has been designed, manufactured and tested in conjunction with the CCHM2.
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Components for laser micromanipulation in a coherence-controlled digital holographic microscope
Straka, Branislav ; Antoš, Martin (referee) ; Dostál, Zbyněk (advisor)
In the Bachelor's thesis, there was described the general principle of operation of the optical trap, along with various options for creating optical tweezers. The function and the main parameters of holographic microscope with the off-axis achromatic and spatially invariant interferometer were described, too. Two variations of laser tweezers connectable to coherence-controlled second generation holographic microscope were described in detail, calculated and designed at IPE VUT. The optimization of the two variations was performed in the software ZEMAX. A 3D model in the Autodesk Inventor PROFESSIONAL 2010 was created for the chosen design.
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