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Screening of biological activity of various species of the genus Narcissus sp. IV
Valešová, Zuzana ; Suchánková, Daniela (advisor) ; Vrabec, Rudolf (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical botany Author: Zuzana Valešová Supervisor: PharmDr. Daniela Suchánková, Ph.D. Title of thesis: Screening of biological activity of various species of the genus Narcissus sp. IV Key words: Narcisus sp., bulbs, alkaloidal extracts, GC/MS analysis, biological activity, acetylcholinesterase, butyrylcholinesterase Plants of the family Amaryllidaceae have a long history of using as traditional remedies. Plants of this family produce structurally unique isoquinoline compounds, which have been called "Amaryllidaceae alkaloids". Over 600 Amaryllidaceae alkaloids have been isolated, which are characterized by interesting biological activity. Cytotoxic, antimicrobial, antiparasitic, anti-inflammatory activity has been described, but the best known is the inhibitory activity against the enzymes acetylcholinesterase and butyrylcholinesterase, which is used in practice for therapy of Alzheimer's disease. [8, 9] The genus Narcissus is primarily known as decorative plants that blooms in early spring. However, daffodils are also used to isolate galanthamine or other alkaloids that are being studied for their effect. [13] The aim of this thesis was to screen the biological activity of extracts of the...
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Screening of biological activity of various species of the genus Narcissus sp. IV
Valešová, Zuzana ; Suchánková, Daniela (advisor) ; Vrabec, Rudolf (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical botany Author: Zuzana Valešová Supervisor: PharmDr. Daniela Suchánková, Ph.D. Title of thesis: Screening of biological activity of various species of the genus Narcissus sp. IV Key words: Narcisus sp., bulbs, alkaloidal extracts, GC/MS analysis, biological activity, acetylcholinesterase, butyrylcholinesterase Plants of the family Amaryllidaceae have a long history of using as traditional remedies. Plants of this family produce structurally unique isoquinoline compounds, which have been called "Amaryllidaceae alkaloids". Over 600 Amaryllidaceae alkaloids have been isolated, which are characterized by interesting biological activity. Cytotoxic, antimicrobial, antiparasitic, anti-inflammatory activity has been described, but the best known is the inhibitory activity against the enzymes acetylcholinesterase and butyrylcholinesterase, which is used in practice for therapy of Alzheimer's disease. [8, 9] The genus Narcissus is primarily known as decorative plants that blooms in early spring. However, daffodils are also used to isolate galanthamine or other alkaloids that are being studied for their effect. [13] The aim of this thesis was to screen the biological activity of extracts of the...
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Screening of biological activity of various species of the genus Narcissus sp. I
Komendová, Karolína ; Suchánková, Daniela (advisor) ; Chlebek, Jakub (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy and Pharmaceutical botany Candidate: Karolína Komendová Supervisor: PharmDr. Daniela Hulcová, Ph.D. Title of thesis: Screening of biological activity of various species of the genus Narcissus sp. I Key words: Amaryllidaceae, Narcissus, alkaloids, AChE, BuChE, Alzheimer's disease Alkaloids from family Amaryllidaceae (AA) nowadays belong to the most important and also the most examined alkaloids. This is because of their structural diversity and a broad spectrum of biological effects. The main aim of my diploma was to prepare whole bulb extracts from seven different cultivated varieties of genus Narcissus and to select those with the highest potential to inhibit enzyme acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Inhibition of these enzymes is very important in the treatment of Alzheimer's disease (AD). Summary extracts were obtained from these cultivars: N. cv, Apotheose (AL - 718), N. cv. Chromacolor (AL - 722), N. cv. Hungarian Rhapsody (AL - 729), N. cv. Mon Cheri (AL - 736), N. cv. Peach Cobbler (AL - 738), N. cv. Pink Pride (AL - 740) a N. cv. Red Devon (AL - 742). The isolated extracts were subsequently analyzed by GC-MS. The following alkaloids were found in the majority of extracts:...
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