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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Prionové proteiny a jejich interakce s těžkými kovy
 Sedláčková, Eliška
Prion proteins are infectious glycoproteins that do not contain nucleic acids. Their specific function in the brain is not yet fully understood. Conversion of the cellular form of prion protein (PrPC) into the pathological isoform (PrPSc) is regarded as the cause of neurodegenerative diseases. PrPC is a glycoprotein that interacts with many divalent metal ions, particularly Cu2+ and Zn2+. Another protein having the ability to bind metals, is also metallothionein (MT), which consists of several specific forms. Regarding formation of neurodegenerative diseases is significant metallothionein isoform MT-3 occuring in the brain. One of the MT-3 functions in the brain is its participation in maintaining of the optimal concentration of metal ions. The aim of this study was to verify the presence of expression PrPC or MT-3 genes in E. coli bacterial cells. Furthermore, we investigated the effect of metal additions on E. coli bacterial culture expressing PrPC or MT-3 protein. In the end we focused on determining the total concentration of MT in E. coli bacterial cells expressing the PrPC or MT-3 protein. Any determination was compared to a standard E. coli BL21 (DE3) strain.
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Detekce prionových proteinů a jejich interakce s kovy a metalothioneinem
 Cardová, Alžběta
Prion diseases are formed by a conformational change of prion-like protein (PrPC) with alfa-helix structure to the pathological isoform - prion (PrPSc) which acquires beta-sheet structure. PrPC physiological properties in the brain are insufficiently described but there is an assumption of its affinity to metal ions. Another protein with metal-binding ability is metallothionein (MT). Brain specific isoform of MT is called MT-III and it is assumed to participate in maintenance of metal ions concentration in the brain. Aim of this study was to prepare recombinant human PrPC in E. coli. Furthermore, this protein was used to detect interactions between metal ions (Cu, Zn), MT and PrPC by differential pulse voltammetry method. The final part was devoted to the MT-III determination in different genotypes of prion-infected and non-infectious mouse brain tissues.
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