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Dermatophytes isolated from the hair of free-living rodents
Žárová, Štěpánka ; Čmoková, Adéla (advisor) ; Mašínová, Tereza (referee)
Dermatophytes (order Onygenales, Ascomycota) are microscopic filamentous keratinophilic fungi that can cause skin infections known as dermatophytosis. The most diverse but not very studied genus Arthroderma has been revised recently (Míková 2018) which was essential for further research. This genus comprises mostly species with a supposed reservoir in soil. Lack of information about their ecology and frequent isolation of some species from the hair of free- living mammals (mainly rodents) may testify a strong host association. Rodents could thus represent the hidden reservoir of this species. For this thesis, I have chosen three ecologically distinct rodent species: Mus musculus, Apodemus flavicollis, and Clethrionomys glareolus. I obtained the material by brushing the hair of asymptomatic individuals and used this material for cultivation on selective medium. I identified the isolates of dermatophytes (n = 30) using molecular methods. I used sequences of three highly variable loci (ITS, tubb a tef1α) to incorporate these isolates in the phylogenetic analysis based on the monography of the genus Arthroderma (Míková 2018). I characterized the phenotype of selected strains based on morphological and physiological data including the ability to utilize keratin and the production of siderophores. The...
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Sexual system of the ascomycetes from order Pezizales
Janošík, Lukáš ; Koukol, Ondřej (advisor) ; Čmoková, Adéla (referee)
Sexual reproduction is essential part of life for numerous ascomycetes. Based on their requirement for opposite partner to produce fruiting bodies with ascospores, fungi can be in general divided into two mating systems - homothallism and heterothallism. However, several recent studies suggest that this division is clearly simplified and homothallism is an umbrella term for diverse sexual behaviours. First part of this thesis is focused on the reproduction, mating systems and their evolution in division Ascomycota. The main part summarizes present knowledge about the mating systems of the fungi from order Pezizales. Observations about the members of individual families are discussed, summed up and reviewed in the light of phylogeny and ecology.
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Dermatophytes isolated from the hair of free-living rodents
Žárová, Štěpánka ; Čmoková, Adéla (advisor) ; Mašínová, Tereza (referee)
Dermatophytes (order Onygenales, Ascomycota) are microscopic filamentous keratinophilic fungi that can cause skin infections known as dermatophytosis. The most diverse but not very studied genus Arthroderma has been revised recently (Míková 2018) which was essential for further research. This genus comprises mostly species with a supposed reservoir in soil. Lack of information about their ecology and frequent isolation of some species from the hair of free- living mammals (mainly rodents) may testify a strong host association. Rodents could thus represent the hidden reservoir of this species. For this thesis, I have chosen three ecologically distinct rodent species: Mus musculus, Apodemus flavicollis, and Clethrionomys glareolus. I obtained the material by brushing the hair of asymptomatic individuals and used this material for cultivation on selective medium. I identified the isolates of dermatophytes (n = 30) using molecular methods. I used sequences of three highly variable loci (ITS, tubb a tef1α) to incorporate these isolates in the phylogenetic analysis based on the monography of the genus Arthroderma (Míková 2018). I characterized the phenotype of selected strains based on morphological and physiological data including the ability to utilize keratin and the production of siderophores. The...
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Sexual system of the ascomycetes from order Pezizales
Janošík, Lukáš ; Koukol, Ondřej (advisor) ; Čmoková, Adéla (referee)
Sexual reproduction is essential part of life for numerous ascomycetes. Based on their requirement for opposite partner to produce fruiting bodies with ascospores, fungi can be in general divided into two mating systems - homothallism and heterothallism. However, several recent studies suggest that this division is clearly simplified and homothallism is an umbrella term for diverse sexual behaviours. First part of this thesis is focused on the reproduction, mating systems and their evolution in division Ascomycota. The main part summarizes present knowledge about the mating systems of the fungi from order Pezizales. Observations about the members of individual families are discussed, summed up and reviewed in the light of phylogeny and ecology.
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