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The influence of physiologically controlled motor activity on biomechanical cross-section properties of long bones.
Jindrová, Alena ; Sládek, Vladimír (advisor) ; Špoutil, František (referee)
The mechanical loading affects the long bone cross-section geometry (CSG). The aim of this thesis was to examine experimentally the relationship between the locomotory parameters and cross-sectional properties of long bones, and to test some partial knowledge of the bone mechanical adaptation. Two groups of B6CBA mice were examined: (a) Lurcher type (model of olivocerebellar degeneration; n = 10) and (b) control (normal mice; n = 10). We analyzed the motor characteristics and the bone cross-sectional geometric properties. The motor tests included the test of spontaneous motor activity (Open field), strength properties (horizontal bar) and motor coordination (rotarod). Cross-sections were taken in 50 % of the left tibia biomechanical length and further processed for fluorescence confocal microscopy. We analyzed the biomechanical properties of cortical bone cross-sections (software ImageJ). There were nonsignificant differences in CSG parameters (TA, CA, Imax, Imin, J, Imax/Imin) between Lurcher and control mice. The results did not support our assumption about the effect of motor disorder on CSG properties. We did not demonstrate the effect of local factors on the bone biomechanical adaptation. The results of this thesis may be useful to find new testing possibilities of cortical bone in...
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Changes motor functions in mouse model of cerebellar degeneration in the course of the ontogenesis
Šalomová, Martina ; Jelínková, Dana (advisor) ; Kubik-Zahorodna, Agnieszka (referee)
The cerebellum affects a number of important and complex processes in the organism. It ensures coordination, motor learning and plays an important role in cognitive and affective functions. In the case of cerebellar degeneration, we find not only the movement disorders but also behavioral abnormalities, collectively referred to as cognitive-affective syndrome. The aim of this work was to investigate motor functions during ontogenesis in animal models of hereditary cerebellar degeneration - mutant mice Lurcher and Purkinje cell degeneration using the device for quantitative gait analysis and rotarod. In addition, the effect of physical activity on the extent of ataxia and manifestations in classical behavioral tests was monitored. The results confirmed significant differences in motor skills between mutant and healthy mice; differences were also observed in some gait parameters, especially in walking speed and parameters that correlate with it. The motor functions of most groups of mice did not change during ontogenesis, pcd mice deteriorated their performance on the rotarod. The effect of physical activity was not found, with the exception of Forced swimming test. Physical activity of set intensity did not have any beneficial effect on the motoric manifestations of the mice.
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The influence of physiologically controlled motor activity on biomechanical cross-section properties of long bones.
Jindrová, Alena ; Sládek, Vladimír (advisor) ; Špoutil, František (referee)
The mechanical loading affects the long bone cross-section geometry (CSG). The aim of this thesis was to examine experimentally the relationship between the locomotory parameters and cross-sectional properties of long bones, and to test some partial knowledge of the bone mechanical adaptation. Two groups of B6CBA mice were examined: (a) Lurcher type (model of olivocerebellar degeneration; n = 10) and (b) control (normal mice; n = 10). We analyzed the motor characteristics and the bone cross-sectional geometric properties. The motor tests included the test of spontaneous motor activity (Open field), strength properties (horizontal bar) and motor coordination (rotarod). Cross-sections were taken in 50 % of the left tibia biomechanical length and further processed for fluorescence confocal microscopy. We analyzed the biomechanical properties of cortical bone cross-sections (software ImageJ). There were nonsignificant differences in CSG parameters (TA, CA, Imax, Imin, J, Imax/Imin) between Lurcher and control mice. The results did not support our assumption about the effect of motor disorder on CSG properties. We did not demonstrate the effect of local factors on the bone biomechanical adaptation. The results of this thesis may be useful to find new testing possibilities of cortical bone in...
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