|
|
Leucocytozoon and methods of its detection in raptors
Sekerášová, Iveta ; Brzoňová, Jana (advisor) ; Majerová, Karolina (referee)
The genus Leucocytozoon is a bird parasite. Its hosts constitute usually of representatives of the Falconiformes group and hawks (Accipitridae). The vector of this parasite are blackflies (family Simuliidae). This thesis brings an overview of species of the genus Leucocytozoon so far detected in birds of prey. The thesis focuses primarily on methods of detecting Leucocytozoon in hosts. Traditionally, this genus has been described on the basis of its morphological characters observed via microscope. With the discovery of polymerase chain reaction method (PCR), the detection possibilities expanded and allow us to study this parasite on molecular levels. The thesis contains a basic overview of detection methods, characteristics of their principles and outlines their strengths and weaknesses regarding the practical applicability as well. Based on that, the microscopic approach alongside with the detection based on molecular methods is recommended.
|
|
|
Leucocytozoon in passerines
Geržová, Lenka ; Svobodová, Milena (advisor) ; Vinkler, Michal (referee)
Leucocytozoon is the least studied genus of blood parasites of the order Haemosporida comparison with the genera Plasmodium and Haemoproteus. Its transmission is almost exclusively linked with a vector of the genus Simulium, which ensures the circulation of parasites in populations of wild and domestic birds. Numerous species have been are described by classical taxonomy, but it is assumed that this genus hides a number of cryptic species, its diversity being much higer. Methods of detection include microscopy, a classical method used for many decades, and molecular biology methods introducted at the turn of the millenium. Key words: Leucocytozoon, passerines, prevalence, species, dynamics of infection, detection
|
|
|
The role of biting midges in avian blood parasites transmission
Chobotová, Kristýna ; Brzoňová, Jana (advisor) ; Kočišová, Alica (referee)
Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are hematophagous in- sects mainly important as vectors of viral diseases of farm animals. They have shifted to the forefront of scientific interest in the Czech Republic primarily in relation to the Bluetongue Virus spread since 2007. However, they have been often ignored as vectors of parasitic infec- tions, especially if these are not pathogens of economic importance. In birds, which are the focus of this bachelor's thesis, biting midges are the most significant vector mainly for the genus Haemoproteus, they transmit at least 24 species of these protozoa. On the other hand, they are proven vectors for only one species of the genus Leucocytozoon - L. caulleryi. Trans- mission has been proven for three species of the genus Trypanosoma - T. avium, T. bennetti and T. everetti. Biting midges also transmit helminths of the family Onchocercidae, they play a role as vectors for the genera Eufilaria, Chandlerella and Splendidofilaria. The goal of this bachelor's thesis is to gather information about biting midges as vectors of avian blood parasites - protozoa of the genera Haemoproteus, Leucocytozoon and Trypanosoma and helminths of the family Onchocercidae. Key words: biting midges, parasite, vector, host, bird, Haemoproteus,...
|
|
|
Leucocytozoon and methods of its detection in raptors
Sekerášová, Iveta ; Brzoňová, Jana (advisor) ; Majerová, Karolina (referee)
The genus Leucocytozoon is a bird parasite. Its hosts constitute usually of representatives of the Falconiformes group and hawks (Accipitridae). The vector of this parasite are blackflies (family Simuliidae). This thesis brings an overview of species of the genus Leucocytozoon so far detected in birds of prey. The thesis focuses primarily on methods of detecting Leucocytozoon in hosts. Traditionally, this genus has been described on the basis of its morphological characters observed via microscope. With the discovery of polymerase chain reaction method (PCR), the detection possibilities expanded and allow us to study this parasite on molecular levels. The thesis contains a basic overview of detection methods, characteristics of their principles and outlines their strengths and weaknesses regarding the practical applicability as well. Based on that, the microscopic approach alongside with the detection based on molecular methods is recommended.
|
guest ::
