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Using tests with destruents for ecotoxicological evaluation of soil quality
Rábová, Petra ; Mravcová, Ludmila (referee) ; Zlámalová Gargošová, Helena (advisor)
The aim of this diploma thesis was to determine the dehydrogenase activity of matrix samples that can enter the environment and the use of ecotoxicological tests to assess the quality of the affected soil. The effects of sludge, biochar samples, two landfill samples and a sediment sample were monitored. Ecotoxicology included tests on decomposers - tests of avoidance behavior (Eisenia fetida and Folsomia candida), acute, chronic and reproductive toxicity tests (Eisenia fetida) and Lactuca sativa root growth inhibition tests. The results indicate that dehydrogenase activity assays serve as a suitable complement to ecotoxicological tests. They provide valuable additional information on soil quality and sample effect after application to soil. The sediment sample had the least favorable effect on the activity of the enzyme and the vitality of the organisms, both in the determination of dehydrogenase activity and in ecotoxicological tests. Furthermore, it was found that biochar as a product of sewage sludge treatment is less toxic to organisms than the original sludge.
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Risks associated with the use of biochar in the terrestrial ecosystem
Valeeva, Adeliia ; Řezáčová, Veronika (referee) ; Zlámalová Gargošová, Helena (advisor)
The aim of this bachelor thesis was to assess the ecotoxicity of biochar produced from sewage sludge in Ingolstadt. Biochar is one of the carbon storage options and it’s applied to the soil. Biochar has an effect not only on the terrestrial ecosystem in direct contact of organisms with the sample, but also on aquatic in the leaching of substances from biochar into water. Therefore, contact and aquatic tests were chosen to assess the ecotoxicological effect. The representative of the decomposers was the earthworm Eisenia fetida, the primary producer – the plant Lactuca Sativa and the Daphnia magna was chosen from the aquatic organisms. The test results differed slightly, but the lowest negative effect on the test organisms was shown by soil samples with the addition of 5 g/kg and 10 g/kg granular biochar.
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Vliv alternativních zdrojů bílkovin v rybím krmivu na růst ryb a rostlin ve dvousmyčkovém akvaponickém systému
NIKL, Ondřej
The objective of this thesis was to evaluate the production efficiency of feeds which were designed with the intention of developing a special aquaponic feed. Further, to evaluate the content of nutrients in water, sludge and nutrient solutions and their influence on plant growth and finally suggest possible modifications to the feed composition. Aquaponic feed should respect nutritional requirements of fishes and plants, not put burden on the environment by not used nutrients and with regards to sustainability, use alternative sources of protein instead of marine fish meal. Because of that, three experimental isoprotein and isocaloric feeds were proposed. Feed A contained conventional ingredients including marine fish meal. Feed B used freshwater fish meal and exclusively plant proteins not regarding its origin. Feed C was made of sustainable and local sources of protein. The experiment was conducted in the aquaponic hall of Faculty of Fisheries and Protection of Waters, University of South Bohemia. As experimental material was used Nile tilapia (Oreochromis niloticus) and lettuce (Lactuca sativa). Feed B shown similar values of all production indicators as conventional feed A. Weight of fishes in system A after thirteen weeks increased by 143,68 % and in system B by 136,56 %. Weight of fishes in system B was at the end of experiment inconclusively lower by 4,08 %. Feed conversion ratio (FCR) of feed B (1,92?0,10) was inconclusively higher by 8,47 % against FCR of feed A (1,77?0,13). Average weight of aerial parts of plants growing in nutrient solution created from mineralized sludge gathered from system B, supplemented with missing nutrients reached value 221,8?33,9 g after four weeks. That was inconclusively more by 44,21 % than average weight of plants grown in special hydroponic solution and by 8,62 % more than weight of plants grown in enriched solution gathered from system A. Experimentally it was proven that in aquaponic feeds it is possible to substitute marine fish meal by alternative source of protein and using these feeds ensure an effective nutrition for fish and plants in a two-loop aquaponic system.
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Risks associated with the use of biochar in the terrestrial ecosystem
Valeeva, Adeliia ; Řezáčová, Veronika (referee) ; Zlámalová Gargošová, Helena (advisor)
The aim of this bachelor thesis was to assess the ecotoxicity of biochar produced from sewage sludge in Ingolstadt. Biochar is one of the carbon storage options and it’s applied to the soil. Biochar has an effect not only on the terrestrial ecosystem in direct contact of organisms with the sample, but also on aquatic in the leaching of substances from biochar into water. Therefore, contact and aquatic tests were chosen to assess the ecotoxicological effect. The representative of the decomposers was the earthworm Eisenia fetida, the primary producer – the plant Lactuca Sativa and the Daphnia magna was chosen from the aquatic organisms. The test results differed slightly, but the lowest negative effect on the test organisms was shown by soil samples with the addition of 5 g/kg and 10 g/kg granular biochar.
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Using tests with destruents for ecotoxicological evaluation of soil quality
Rábová, Petra ; Mravcová, Ludmila (referee) ; Zlámalová Gargošová, Helena (advisor)
The aim of this diploma thesis was to determine the dehydrogenase activity of matrix samples that can enter the environment and the use of ecotoxicological tests to assess the quality of the affected soil. The effects of sludge, biochar samples, two landfill samples and a sediment sample were monitored. Ecotoxicology included tests on decomposers - tests of avoidance behavior (Eisenia fetida and Folsomia candida), acute, chronic and reproductive toxicity tests (Eisenia fetida) and Lactuca sativa root growth inhibition tests. The results indicate that dehydrogenase activity assays serve as a suitable complement to ecotoxicological tests. They provide valuable additional information on soil quality and sample effect after application to soil. The sediment sample had the least favorable effect on the activity of the enzyme and the vitality of the organisms, both in the determination of dehydrogenase activity and in ecotoxicological tests. Furthermore, it was found that biochar as a product of sewage sludge treatment is less toxic to organisms than the original sludge.
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