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Diversity of Neofabraea species causing bull's eye rot in the Czech Republic
Pešicová, Kamila ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Neofabraea is a genus of an important plant pathogenic fungi having worldwide appearance. Four Neofabraea species are responsible for bull's eye rot of pome fruits. The aim of this thesis was to investigate which of these species occur in the Czech Republic. 81 isolates were collected during a two- year period and they were identified using PCR fingerprinting (primers ERIC 1R and M13-core) and DNA sequencing (ITS, mtSSU and tub2). The results showed that species N. alba, N. perennans and Cryptosporiopsis kienholzii occur in the Czech Republic. According to available information, this is the second record of C. kienholzii in Europe. One isolate (KP4) failed to be identified as any of the species. KP4 is very close to C. kienholzii, but it can be distinguished both biologically and genetically. Furthermore, the aggressiveness of individual species was compared.N. perennans and strain KP4 proved to be most aggressive, the least aggressive is C. kienholzii. Two N. alba strains (KP36 and KP37) isolated from healthy apple fruit and leaf are pathogenic for apple fruits. Keywords: aggressiveness, Cryptosporiopsis kienholzii, Dermateaceae, Helotiales, apple tree, Malus, PCR fingerprinting, postharvest diseases Powered by TCPDF (www.tcpdf.org)
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Hyaloscyphaceae - current view on phylogeny and diverzity
Bundová, Kateřina ; Koukol, Ondřej (advisor) ; Šandová, Markéta (referee)
The family Hyaloscyphaceae (Helotiales) includes microscopic species of fungi characterized by sessile apothecium with excipular hairs; these hairs are very important in taxonomy of the group. Most species of the family are saprobes and these saprobic species grow on herbaceous or woody litter, most often they grow on fallen needles, cones and stems of coniferous wood. Distribution of the family is cosmopolitan and has center of occurence in temperate and boreal forests, however members of the family also occur in tropic and subtropic forests. This family is probably a polyphyletic group of several lineages and needs to be analyzed further. The aim of this bachelor's thesis is to organize and compare the views on phylogeny of Hyaloscyphaceae, from the first morphological studies until the current molecular studies, and then to summarize all the genera of fungi which belong to this family. And to describe ecological and species diversity.
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Diversity of Neofabraea species causing bull's eye rot in the Czech Republic
Pešicová, Kamila ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Neofabraea is a genus of an important plant pathogenic fungi having worldwide appearance. Four Neofabraea species are responsible for bull's eye rot of pome fruits. The aim of this thesis was to investigate which of these species occur in the Czech Republic. 81 isolates were collected during a two- year period and they were identified using PCR fingerprinting (primers ERIC 1R and M13-core) and DNA sequencing (ITS, mtSSU and tub2). The results showed that species N. alba, N. perennans and Cryptosporiopsis kienholzii occur in the Czech Republic. According to available information, this is the second record of C. kienholzii in Europe. One isolate (KP4) failed to be identified as any of the species. KP4 is very close to C. kienholzii, but it can be distinguished both biologically and genetically. Furthermore, the aggressiveness of individual species was compared.N. perennans and strain KP4 proved to be most aggressive, the least aggressive is C. kienholzii. Two N. alba strains (KP36 and KP37) isolated from healthy apple fruit and leaf are pathogenic for apple fruits. Keywords: aggressiveness, Cryptosporiopsis kienholzii, Dermateaceae, Helotiales, apple tree, Malus, PCR fingerprinting, postharvest diseases Powered by TCPDF (www.tcpdf.org)
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