|
|
Effects of anaesthetics on barbel
ŘEŽÁBEK, Josef
The aim of this study was to assess the effects of the four most used anaesthetics in European aquaculture MS 222, clove oil, 2-phenoxyethanol and Propiscin on barbel (Barbus barbus). The effects of anaesthetics were assessed based on haematological profile, biomarker of oxidative stress and antioxidant enzymes. This study contributed to the expansion of knowledge on the safety of tested anaesthetics and selected safe anaesthetics for barbel. Barbels were exposed to a 10-minute anaesthesia with MS 222 (in recommended concentration 100 mg×l-1), clove oil (in recommended concentration 33 mg×l-1), 2-phenoxyethanol (in recommended concentration 0.4 ml×l-1) and Propiscin (in recommended concentration 1 ml×l-1). The effects of anaesthetics were evaluated immediately after 10 min. anaesthesia and 24 hours after anaesthesia. Anaesthesia with MS 222, clove oil, 2-phenoxyethanol and Propiscin had no significant effect on haematological indices, level of oxidative stress (TBARS) and activity of glutathione reductase in barbel tissues. The activity of catalase was significantly increased in the muscle 24 h after anaesthesia of all anaesthetics compared to the control. Superoxide dismutase activity was changes in all experimental groups (immediately after 10 min. anaesthesia and 24 hours after anaesthesia). The tested anaesthetics not altered hematopoietic tissue and had not effect on the level of lipid peroxidation in barbel´s tissues. The results of this study suggest that the antioxidant systems of barbel are altered by Propiscin anaesthesia, but they are slightly affected by MS 222, clove oil, and 2-phenoxyethanol anaesthesia. On the basis of the results of this thesis, for anaesthesia of barbel we can recommend clove oil and 2-phenoxyethanol as an alternative MS 222.
|
|
|
Comparison of the effectiveness and efficiency of the four common anesthetic used in fisheries for burbot (Lota lota L.)
RŮŽEK, Martin
The aim of this study was to compare the effect of four anaesthetics on haematological and biochemical blood profiles in burbot (Lota lota L.). Both blood profiles of burbot were evaluated 10 min and 24 h after anaesthesia with MS-222 (100 mg.L-1), clove oil (33 mg.L-1), 2-phenoxyethanol (0.30 ml.L-1), Propiscin (1 ml.L-1) and were compared to non-anaesthetised control. The tested anaesthetics did not have any effect on haematological profile of burbot. The exposure to clove oil, 2-phenoxyethanol and Propiscin significantly influenced level of ammonia and glucose. The level of lactate was significantly increased following anaesthesia with 2-phenoxyethanol and Propiscin. The levels of total protein, aspartate aminotransferase and calcium were higher with clove oil, 2-phenoxyethanol and Propiscin compare to control. The use of MS-222 showed the smallest effect on haematological and biochemical blood profile and is recommended as a suitable anaesthetic for burbot.
|
|
|
Effect of anaestetics on fish - review
MALÝ, Jan
Bachelor thesis "Effect of anaestetics on fish" has been written as a review. The aim was to elaborate and create the review about the effect of anaestetics on fish. There can be found effects of 12 widely used anaestecics in the European and world aquacultural regions. I have focused mainly on the four mostly used anaestetics in the Czech aquaculture, especially on the clove oil, MS-222, 2-phenoxyethanol and propiscin. To work properly out this bachelor thesis, I have used home and foreign sources found in the scientis database. My bachelor´s thesis is bringing a complete summary concerning the effect of anaestetics on fish and that is why it can be used for similar surveys (purpose) in the field of fish anaesthesia, other use of anaestecics in aquaculture and fish health protection in accordance with the Animal protection law Nr. 359/2012, which has replaced the former law Nr. 246/1992.
|
|
|
Sensitivity to anesthetic clove oil different species of adult fish
ŠKEŘÍK, Jindřich
Nomenclature: Sensitivity to anesthetic clove oil different species of adult fish Sensitivity of breeding fishes of various species (Siberian sturgeon, Brook trout, Black carp- young breeding fishes) for anesthetic clove oil was probed by the tests performed at temperature in the period of stripping (apart from Brook trout and Black carp). The tests were carried out with 17 fish species- with cyprinids (Ide- golden form, Common dace, European Chub, Sneep, Barbel, Grass carp, Black carp, bighead carp), salmonids (Brown trout, Rainbow trout, Brook trout), thymallinae (Grayling), pikes (Northern pike), perches (Pike-perch), sturgeons (Siberian sturgeon, Beluga) and airbreathing catfishes (North African catfish). Using concentration of 0,03ml.l-1 (Brown trout, Rainbow trout, Brook trout, Grayling, Pike, Pike perch, Ide, Dace, Chub, Barbel and Sneep), 0,04ml.l-1 (Grass carp, Black carp, Bighead carp), 0,05ml.l-1 (North African catfish) and 0,07ml.l-1 (Siberian sturgeon, Beluga) the IIb. Phase has been achieved, which is sufficient for manipulation with fishes during stripping. The anesthesia subsided after time period of 10-17 minutes (12 species) at the majority of fishes and at others after interval of 20-26 minutes (5 species). The results confirmed concentrations recommended for salmodis and Grayling (0,025-0,03ml.l-1), sturgeons (0,07ml.l-1), and North African catfish (0,05ml.l-1). At the majority of cyprinids (apart from Grass carp, Black carp and Bighead carp), the concentration used was lower than recommended (0,04-0,05ml.l-1)
|
guest ::
