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Beta-lactamase positive Haemophilus influenzae in children
BROMOVÁ, Pavla
This Bachelor thesis, called {\crqq}Beta-lactamase Positive Haemophilus influenzae in Children`` deals with the basic properties and current occurrence of the bacteria Haemophilus influenzae, and also with the occurrence or other hemofils (Haemophilus species) in children, which occurred in the biological material sent to the microbiological laboratory- Laboma s.r.o. in České Budějovice, where some of them were producers of {$\beta$}-lactamase. The occurrence of these enzymes might be a serious problem for the right choice of antibiotics for the treatment of diseases caused by these hemofils. The essential objective was to collect and assess all data about {$\beta$}-lactamase positive hemofils in the material from airways of children from birth to 15 years of age and with a certain numbers of diagnoses in the aforementioned microbiological laboratory. The data for my thesis was collected in the second half of 2008 and first half of 2009 and was later statistically processed into graphic figures and charts in the form of results. I have also learned to use an easy and used diagnostic method for the verification of {$\beta$}-lactamase ({$\beta$}-LACTAstrip test), during which the color was changed in the zone of the indicator paper if {$\beta$}-lactamase was present. I have also visited pediatricians, who co-operate with this laboratory. They were willing to reply to four questions in my questionnaire. The main objective of these questions was to focus on the treatment of ill children whose biological material from upper airways shows present {$\beta$}-lactamase positive hemofils. From the collected data I managed to reject hypothesis No. 1 by calculating the average relative quantity from the total occurrence of hemofils producing {$\beta$}-lactamase, and I have found that the production of these enzymes is lower by 50 %. Then, I confirmed hypothesis No. 2 that more common producers of {$\beta$}-lactamase are other hemofils (H. species) than H. influenzae. From replies provided by pediatricians, I found out that all of these pediatricians take the presence of {$\beta$}-lactamase into consideration when they chose antibiotics and this confirmed hypothesis No. 3. This Bachelor thesis can be used as an argument for pediatricians to take into consideration the presence of {$\beta$}-lactamase in the biological material of producers, when they choose antibiotics.
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