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Determination of zearalenone in malting barley
Wawroszová, Simona ; Čumová,, Martina (referee) ; Běláková, Sylvie (advisor)
This bachelor thesis deals with monitoring of zearalenone content in malting barley using immunochemical method ELISA and consequently method of liquid chromatography with mass spectrometry (LC-MS/MS). Theoretical part describes the brewing raw materials and important toxinogenic filamentous fungi. Special attention was drawn to selected fusarium mycotoxins (fumonisins, trichothecenes, zearalenone). It also describes some methods for determination of mycotoxins. Experimental section describes analyze 90 samples of malting barley of different varieties using method ELISA. In 19 samples zearalenone concentration was higher than limit of quantification. The highest level of zearalenone concentration (39,2 g•kg-1) contained barley, variety Wintmalt, where corn was used as a fore-crop. The samples were subsequently analyzed by LC-MS/MS. Zearalenone concentration was higher than limit of quantification only in four samples, namely in samples of Blaník, Malz and Wintmalt varieties of barley. Furthermore, the samples of intermediate products of malting process were analyzed by LC-MS/MS. No sample showed level of zearalenone higher than limit of quantification.
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Determination of zearalenone in malting barley
Wawroszová, Simona ; Čumová,, Martina (referee) ; Běláková, Sylvie (advisor)
This bachelor thesis deals with monitoring of zearalenone content in malting barley using immunochemical method ELISA and consequently method of liquid chromatography with mass spectrometry (LC-MS/MS). Theoretical part describes the brewing raw materials and important toxinogenic filamentous fungi. Special attention was drawn to selected fusarium mycotoxins (fumonisins, trichothecenes, zearalenone). It also describes some methods for determination of mycotoxins. Experimental section describes analyze 90 samples of malting barley of different varieties using method ELISA. In 19 samples zearalenone concentration was higher than limit of quantification. The highest level of zearalenone concentration (39,2 g•kg-1) contained barley, variety Wintmalt, where corn was used as a fore-crop. The samples were subsequently analyzed by LC-MS/MS. Zearalenone concentration was higher than limit of quantification only in four samples, namely in samples of Blaník, Malz and Wintmalt varieties of barley. Furthermore, the samples of intermediate products of malting process were analyzed by LC-MS/MS. No sample showed level of zearalenone higher than limit of quantification.
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