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Preparation of luminescent nanoparticles for 3D imaging
Smolka, Rastislav ; Hrabal, Michal (referee) ; Vala, Martin (advisor)
The aim of this bachelor thesis was to study a suitable methodology for the preparation of fluminiscent nanoparticles and to characterize their optical properties associated with various physical properties of environment. Also, their potential use as a means of monitoring biological preparations in 3D microscopy using multiphoton absorption. In particular, we deal with nanoparticles prepared from N,N-diphenylamino-2,5-diphenyl-1,4-distyrylbenzene cyanide and their fluorescence and absorption spectra. Using the dynamic light scattering method and fluorescence spectroscopy, the dependence of the size of the prepared nanoparticles, the change in their fluorescence properties and the change in the quantum yield is observed, depending on the changing environmental properties. Data from multifoton absorption measurements demonstrate that this substance responds to multifoton excitation. It has been shown that this substance retains its unique fluorescence properties also in the form of nanoparticles and thus appears to be a suitable candidate for the observation of biological preparations using multifoton fluorescence microscopy. Therefore, the continuation of this work could be the mere determination of the total multifoton absorption cross section of these prepared nanoparticles.
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Luminescent nanoparticles for 3D imaging
Smolka, Rastislav ; Mravec, Filip (referee) ; Vala, Martin (advisor)
The aim of this diploma thesis is to study the optical properties of new -conjugated molecules based on 1,4-di(4'-N, N-diphenylaminostyryl)benzene and their potential application in advanced imaging techniques of biological specimens, the so-called multiphoton microscopy. The thesis focuses mainly on the characterization of their optical properties and the determination of their two-photon absorption cross-section using a unique laser equipment. Furthermore, a suitable methodology for the preparation of nanoparticles from these molecules, their characterization and stability are also developed. The thesis also investigates the influence of structure on the optical properties of these molecules. The relation between the length of the conjugated system and the presence of substituents on the backbone has been shown for the optical properties of the molecules in the solvent, the position and shape of the two-photon absorption spectrum and the value of two-photon absorption cross-section. It has been shown that this substance retains its unique fluorescent properties even in the form of nanoparticles and therefore appears to be a suitable candidate for the observation of biological specimens using multiphoton fluorescence microscopy. The work contributes to the knowledge base for the design of the chemical structure of molecules with desired properties.
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Luminescent nanoparticles for 3D imaging
Smolka, Rastislav ; Mravec, Filip (referee) ; Vala, Martin (advisor)
The aim of this diploma thesis is to study the optical properties of new -conjugated molecules based on 1,4-di(4'-N, N-diphenylaminostyryl)benzene and their potential application in advanced imaging techniques of biological specimens, the so-called multiphoton microscopy. The thesis focuses mainly on the characterization of their optical properties and the determination of their two-photon absorption cross-section using a unique laser equipment. Furthermore, a suitable methodology for the preparation of nanoparticles from these molecules, their characterization and stability are also developed. The thesis also investigates the influence of structure on the optical properties of these molecules. The relation between the length of the conjugated system and the presence of substituents on the backbone has been shown for the optical properties of the molecules in the solvent, the position and shape of the two-photon absorption spectrum and the value of two-photon absorption cross-section. It has been shown that this substance retains its unique fluorescent properties even in the form of nanoparticles and therefore appears to be a suitable candidate for the observation of biological specimens using multiphoton fluorescence microscopy. The work contributes to the knowledge base for the design of the chemical structure of molecules with desired properties.
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Preparation of luminescent nanoparticles for 3D imaging
Smolka, Rastislav ; Hrabal, Michal (referee) ; Vala, Martin (advisor)
The aim of this bachelor thesis was to study a suitable methodology for the preparation of fluminiscent nanoparticles and to characterize their optical properties associated with various physical properties of environment. Also, their potential use as a means of monitoring biological preparations in 3D microscopy using multiphoton absorption. In particular, we deal with nanoparticles prepared from N,N-diphenylamino-2,5-diphenyl-1,4-distyrylbenzene cyanide and their fluorescence and absorption spectra. Using the dynamic light scattering method and fluorescence spectroscopy, the dependence of the size of the prepared nanoparticles, the change in their fluorescence properties and the change in the quantum yield is observed, depending on the changing environmental properties. Data from multifoton absorption measurements demonstrate that this substance responds to multifoton excitation. It has been shown that this substance retains its unique fluorescence properties also in the form of nanoparticles and thus appears to be a suitable candidate for the observation of biological preparations using multifoton fluorescence microscopy. Therefore, the continuation of this work could be the mere determination of the total multifoton absorption cross section of these prepared nanoparticles.
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