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Annotated translation: 2 kap. in WILCOX, R. V. - COCHRAN, T. R., Investigating Firefly and Serenity: science fiction on the frontier. London: I.B. Tauris, 2008. Ss. 53-62, 89-100.
Exnerová, Nika ; Kalivodová, Eva (advisor) ; Eliáš, Petr (referee)
The focus of this work is the translation of two articles from Investigating Firefly and Serenity: Science Fiction on the Frontier, an anthology of essays and articles from various authors and researches specializing in the humanities. The two selected articles focus on dystopic tradition and feminism. An annotation of the translation from the perspective of translation theory forms the second part of this work: it contains the original text analysis based on the model of Christiane Nord, and describes the translation method and problems as well as their solutions and the typology of translation shifts. Examples from both the original and the translation can be found in the text. Powered by TCPDF (www.tcpdf.org)
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Cysteine tRNA regulates protein synthesis in human cell lines
Kučerová, Michaela ; Beznosková, Petra (advisor) ; Půta, František (referee)
A significant number of known human genetic diseases is associated with nonsense mutations leading to the introduction of a premature termination codon into the coding sequence. A termination codon can be read through by its near-cognate tRNA (tRNA with two anticodon nucleotides base-pairing with a stop codon); potentially generating C-terminally extended protein variants. In yeast, UGA stop codon was described to be read through by tRNA-Trp and tRNA-Cys. Similar was observed for tRNA-Trp in human HEK293T cell line. The aim of this thesis was to investigate if human tRNA-Cys can act as a near-cognate tRNA in human HEK293T cell line. There are two isoacceptors which constitute the tRNA-Cys family, with ACA and GCA anticodon. There are 1 and 23 isodecoders to the ACA and GCA anticodons, respectively. Here, altogether as many as nine tRNA-Cys isodecoders (distinct in their sequence and with varying levels of expression) were tested for their ability to increase UGA readthrough in HEK293T using p2luci and pSGDluc dual-luciferase reporter vectors. In both p2luci and pSGDluc, we observed that at least one tRNA-Cys isodecoder, tRNA-Cys-GCA-4-1, is capable of significantly elevating the UGA readthrough levels when overexpressed in HEK293T. This indicates that similarly to yeast, tRNA-Cys is capable of...
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Annotated translation: 2 kap. in WILCOX, R. V. - COCHRAN, T. R., Investigating Firefly and Serenity: science fiction on the frontier. London: I.B. Tauris, 2008. Ss. 53-62, 89-100.
Exnerová, Nika ; Kalivodová, Eva (advisor) ; Eliáš, Petr (referee)
The focus of this work is the translation of two articles from Investigating Firefly and Serenity: Science Fiction on the Frontier, an anthology of essays and articles from various authors and researches specializing in the humanities. The two selected articles focus on dystopic tradition and feminism. An annotation of the translation from the perspective of translation theory forms the second part of this work: it contains the original text analysis based on the model of Christiane Nord, and describes the translation method and problems as well as their solutions and the typology of translation shifts. Examples from both the original and the translation can be found in the text. Powered by TCPDF (www.tcpdf.org)
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