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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Enzyme hydrolysis of potato proteins and possibilities of fractionation of obtained peptide fragments
 MIKOVÁ, Klára
The diploma thesis is focused on enzyme hydrolysis of potato protein concentrates and fractionation of obtained peptide fragments. Were used protein concentrate from tubers variety Ornella and protein concentrate obtained by swedish company Lyckeby Starch AB. The enzyme hydrolysis lasted 24 hours and were used the proteolytic enzyme alkalasa and trypsin. In this work were prove possitive effect of enzyme hydrolysis on solubility and antioxidative properties of potato protein isolates. The fractionation of obtained peptide hydrolysated was based on systém FPLC (Fast protein liquid chromatography). The fractions contained of peptide fragments about 1, 350 kDa or fragments of smaller moleculary weight. The antixodative activity of subfractions were determIne by method called DPPH. The highest values (2,2 and 2,6 TEAC g/kg) were accured at the subfractions which were separations from Ornella hydrolyzates digeste by enzyme alkalasa.
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Separation of selected acidic proteins fractions from potato (Solanum tuberosum L.) tubers by chromatographic techniques
 LORENC, František
This diploma thesis deals with acidic proteins contained in the potato (Solanum tuberosum) tubers, or rather about their separation with chromatography techniques. For the analysis were chosen tubers of two czech potato varieties, Adéla and Westamyl. From the concentrated potato juice were eliminated basic and most of the patatin proteins with the gravity column chromatography. In the next step were applied the chromatography techniques on the anion and hydroxyapatite columns by the fast protein liquid chromatography (FPLC) system. On the anion column were separated protein fractions which contained proteins of molecular weight in the range of 15 kDa to 60 kDa (Adéla) and 15 kDa to 100 kDa (Westamyl). The most abundant were the proteins with molecular weight 15 kDa a 20 kDa. In the last step was used the mass spectrometry for the identification of chosen protein fractions.
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Effects of cadmium on the metabolism of wiid-roting fungi: induction of -SH groups and formation of sulphide
 Gogelová, Helena ; Frolík, Jan ; Baldrian, Petr ; Gabriel, Jiří
Stress response of wood-rptting fungi to heavy metals comprises among others reduction of growth rate.
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