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Mutant p53 protein and its binding and transactivation properties
Vojsovič, Matúš ; Němcová, Andrea (referee) ; Brázda, Václav (advisor)
The "genome guardian" protein p53 plays an important role in cancer growth. P53 mutations occur in more than 50 % of human cancers. Mutated proteins significantly affect the proper functioning of cells. Due to the mutation, proteins can gain, but also lose, some of their functions, which also help them in modulating cell metabolism. Mutant forms of p53 may be involved in indirect binding or direct binding to DNA. They appeared to have a lower binding activity to the DNA than non-mutated p53. The experimental part of the thesis focuses on measuring the binding properties of selected p53 mutants using gel retardation analysis and using an atomic force microscope and monitoring the transactivation potential. The results were compared with the wild-type form of p53. It has been found that binding to the most common types of local DNA structures reduces the binding activity of p53 mutants over the wild-type. P53 mutants has been shown to have a lower intensity of transactivation than the wild-type p53 by studying their transactivation abilities and also they are able to reduce the intensity of transactivation when co-expressed with p53.
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Mutant p53 protein and its binding and transactivation properties
Vojsovič, Matúš ; Němcová, Andrea (referee) ; Brázda, Václav (advisor)
The "genome guardian" protein p53 plays an important role in cancer growth. P53 mutations occur in more than 50 % of human cancers. Mutated proteins significantly affect the proper functioning of cells. Due to the mutation, proteins can gain, but also lose, some of their functions, which also help them in modulating cell metabolism. Mutant forms of p53 may be involved in indirect binding or direct binding to DNA. They appeared to have a lower binding activity to the DNA than non-mutated p53. The experimental part of the thesis focuses on measuring the binding properties of selected p53 mutants using gel retardation analysis and using an atomic force microscope and monitoring the transactivation potential. The results were compared with the wild-type form of p53. It has been found that binding to the most common types of local DNA structures reduces the binding activity of p53 mutants over the wild-type. P53 mutants has been shown to have a lower intensity of transactivation than the wild-type p53 by studying their transactivation abilities and also they are able to reduce the intensity of transactivation when co-expressed with p53.
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English modal verbs - EMSA update
Škardová, Zuzana ; Šaldová, Pavlína (advisor) ; Malá, Markéta (referee)
This diploma thesis aims to contribute to the proposed project concerning the update of the multi-media application EMSA (Elektronická mluvnice současné angličtiny, The electronic grammar of contemporary English). This project is based on the text of Mluvnice současné angličtiny na pozadí češtiny [Grammar of contemporary English against the background of Czech] by Professor PhDr. Libuše Dušková, DrSc. et al., which was digitalized and can be accessed at the website www.mluvniceanglictiny.cz. In the future, this application aims to contain supplementary texts, relevant bibliographic information, exercises, authentic examples acquired from electronic corpora, etc. The particular task of this diploma thesis is the critical revision of chapter 8.4 Modal verbs. The first step was the survey of relevant literature and identification of parts which are not explicitly covered by Dušková's original text. These findings are presented in the theoretical part of the thesis (chapter 1.); they are concerned mainly with theoretical definitions (e.g. the delimitation of central and marginal modals or kinds of modality) and information concerning the distribution and recent developments of modal verbs. The second main step was to compile these findings into texts which would be suitable for the use in the project...
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