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Molecular analysis of the mitochondrial genom of \kur{Diuraphis noxia} (Aphididae)
CHUNDELOVÁ, Daniela
The complete sequence of mitochondrial DNA from Diuraphis noxia was obtained and characterized. The mitogenome contains a standard set of 13 protein-coding genes, 19 tRNA genes, 2 ribosomal RNA genes. A+T-rich and ?repets? regions in the same order as those of the other analyzed aphids. Comparison to mtDNAs from other Sternorrhyncha species obtained from GenBank revealed possible markers for studies on population differentiation. Phylogenetic analysis using parsimony and maximum likelihood confirmed the classification of Diuraphis noxia into the Aphididae.
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Genetic variability of natural populations of the \kur{Diuraphis noxia} (Aphididae)
SÁBOVÁ, Michala
Genetic variability of natural populations of the species Diuraphis noxia (Aphididae) was studied with RAPD method. First goal was to establish the chosen method in the laboratory and to optimize conditions of RAPD-PCR for D. noxia. This was followed by the pilot study, in which 12 populations were analyzed. Expected variability among individual populations and within population was acknowledged. The results of this investigation came up to the noted progress of diffusion of this species around the World. Results will be used in further more complex study of D. noxia.
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Fylogeography and genetics variability of \kur{Diuraphis noxia} (\kur{Aphididae})
PAŠÍKOVSKÝ, Jiří
The aim of this work was a research of the genetic variability of natural populations of Russian wheat aphid Diuraphis noxia (Aphididae) by means of microsatellite markers and markers for EPIC-PCR. First goal was to introduce the methods and optimise them for Diuraphis noxia. In the follow-up pilot study, specimens from 47 lines representing 12 populations from all over the world were analysed. Having used microsatellite markers, I proved expected variability among individual populations and within them. The highest genetic variability was detected between Chile and Algeria using markers for cytochrome C in EPIC-PCR. These findings can be used for further studies of the genetic variability of the aphid Diuraphis noxia.
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Optimization of cytogenetic techniques in a model aphid, \kur{Diuraphis noxia} (Aphididae)
VESELÁ, Jana
Several cytogenetical methods for investigation of karyotyp were used in an aphid species, Diuraphis noxia (Hemiptera: Aphididae). These methods (FISH with telomeric probe, FISH with rDNA probe, FISH with histone probe and silver staining) were optimized for application to a sister group Adelgidae, where they could be useful for better differentiation of species in particular stages of their complicated life cycle.
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