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Dietary habits of children from age 6 to 12 suffering from diabetes mellitus type 1
ŠUBOVÁ, Aneta
This bachelor's thesis I focuses on the topic of Dietary habits of children from age 6 to 12 suffering from diabetes mellitus type 1. It is based around this group of children because they will be living with diabetes their whole life and as they are getting more independent at this age, it is necessary to teach them as much as possible. The theoretical part of this thesis includes the description of diabetes mellitus itself. Then it focuses on type 1 diabetes only because this type occurs amongst children the most. The theoretical part then contains information about how to prevent type 1 diabetes, how to diagnose it, what complications can occur and possible treatments. There is also an in-depth description of proper eating habits of people suffering from this disease. The end of the theoretical part is dedicated to characterization of 'young school age', dieting of young children and school catering. Aims of this bachelor's thesis were: to map eating habits of children with diabetes mellitus type 1, to compare them to eating habits of healthy children and to map physical activity of children suffering from diabetes. The research involved 10 children - half of them suffering from type 1 diabetes, the other half were healthy children. Data were obtained in an interview, through a survey and by listing the food intake. It appears that children suffering from type 1 diabetes tend to have more variety in their diet, they consume some kind of food (e.g. vegetables and fruit) more often and they also have higher fiber intake than healthy children. Analysing the food intake revealed lower average intake of fats and energy amongst children who suffer from diabetes. The amount of fats consumed by children with diabetes corresponded with recommendations. But, according to recommendations, energy intake was not correct in either one of these groups of children.
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Impact of mesenchymal stem cells on islets revascularization after transplantation into the extracellular matrix
Hudzieczková, Aneta ; Girman, Peter (advisor) ; Černý, Jan (referee)
Pancreatic transplantation is the only possible treatment to induce independence from exogenous insulin administration in type 1 diabetes mellitus. However, the shortage of donor organs remains the main limitation of pancreas transplantations. The goal of the research is the preparation of a bioartificial organ based on cell therapy. Parts of the extracellular matrix obtained by decellularization of the pancreas are used for its preparation. The protein scaffolds prepared in this way are then repopulated by different cell types again. The extracellular matrix provides structural support to cells, mediates signaling for differentiation, proliferation or migration. Mesenchymal stromal cells are used in clinical therapy, have a positive effect on tissue regeneration processes, modulating the function of the extracellular matrix, suppress inflammation and promote angiogenesis. After pancreas decellularization, we repopulated the extracellular matrix with islets, mesenchymal cells and endothelial cells. Then, the pancreas was transplanted subcutaneously into syngeneic diabetic rats to observe islet revascularization. Based on sections of explanted scaffolds, we found out that revascularization of the islets was higher without the endothelial cells in the transplanted extracellular matrix. Key words:...
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Diabetes mellitus versus celiac disease, genetic testing of the above mentioned diseases using the Protrans kit
PARÝZKOVÁ, Barbora
Celiac disease is an autoimmune disease affecting the mucous membrane of small intestine. The development of this disease is affected by genetic predispositions, but the onset of this disease is also influenced by environmental factors. Genetic predisposition is primarily attributed to HLA system, HLA-DQ2 and HLA-DQ8 in particular. Celiac disease is characterized by many symptoms therefore its diagnosis is difficult and stays undiagnosed in many cases. The aim of the theoretical part of this bachelor´s thesis was to summarize the knowledge of this topic regarding celiac disease and diabetes type 1. as an associated disease, possibly coexisting with celiac disease. The work, dealt with celiac disease occurrence, its pathogenesis, symptoms and types and possible diagnostic approaches and effective treatment. In the end of the theoretical part it also mentions possible associated diseases with celiac disease. The practical part of my work took part in the genetic laboratory GENLABS, where the examination of genetic predispositions to celiac disease were conducted, based on HLA typing using commercially available Protrans kit. The aim of the practical part was to master the diagnostic method of determining the risk HLA haplotype. Further, mastering the DNA isolation method, using peripheral blood and buccal smear, preparation and execution of Polymerase Chain Reaction (PCR) and detection of created PCR products using gel electrophoresis on agarose gel. The Protrans kit is not used for determination of the predispositions to celiac disease in GENLABS any more, it was replaced by real-time PCR method, which is less burdensome and faster and less financially demanding, than the previous one.
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