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Use of autotrophic algae to production of biomass and some metabolites
Chrástová, Nikola ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
Chlorophyll and carotenoids are natural pigments and antioxidants. These pigments are suitable for use in the pharmaceutical, cosmetics or food industries. Lipids are another major metabolite of algae and are a potential feedstock for biofuel production. This bachelor thesis deals with the cultivation of selected genera of autotrophic algae, subsequent isolation of these selected metabolites and their application. The theoretical part focuses on the description of the algae, the metabolites produced, the cultivation and the chosen methods. The experimental part deals with the optimization of the cultivation of algae of the genera Chlorella, Chlamydomonas and Desmodesmus, depending on the chosen medium and intensity of illumination, determination and quantification of algal biomass. Further, extraction of lipidic and carotenoid substances and determination by HPLC and GC was performed. In the overall comparison, the best producer of biomass is the algal genus Desmodesmus and the highest production of pigments and lipid substances was reached in algae of the genus Chlamydomonas.
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Use of autotrophic algae to production of biomass and some metabolites
Chrástová, Nikola ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
Chlorophyll and carotenoids are natural pigments and antioxidants. These pigments are suitable for use in the pharmaceutical, cosmetics or food industries. Lipids are another major metabolite of algae and are a potential feedstock for biofuel production. This bachelor thesis deals with the cultivation of selected genera of autotrophic algae, subsequent isolation of these selected metabolites and their application. The theoretical part focuses on the description of the algae, the metabolites produced, the cultivation and the chosen methods. The experimental part deals with the optimization of the cultivation of algae of the genera Chlorella, Chlamydomonas and Desmodesmus, depending on the chosen medium and intensity of illumination, determination and quantification of algal biomass. Further, extraction of lipidic and carotenoid substances and determination by HPLC and GC was performed. In the overall comparison, the best producer of biomass is the algal genus Desmodesmus and the highest production of pigments and lipid substances was reached in algae of the genus Chlamydomonas.
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