|
|
Evaluation of numerical representations suitability for overlap detection
Pleskačová, Barbora ; Maděránková, Denisa (referee) ; Jugas, Robin (advisor)
The bachelor´s thesis is focused on the evaluation of numerical representations suitability for overlap detection. Introductory part deals with description of deoxyribonucleic acid structure. The next part discribes sequencing methods and genome assembly techniques. Following part deals with numerical representations that convert DNA sequences into numerical form. Based on similarity metrics, the use of these representations is tested for the detection of overlaps between DNA reads. In the practical part of the thesis an algorithm for overlap detection is designed and implemented using numerical representations. The algorithm is then tested on data.
|
|
|
Genetická diverzita zástupců čeledi sezamovitých Pedaliaceae R.Br.
Šlampa, Vojtěch
The family Pedaliaceae belonging to the order Lamiales, described in 1810 by Robert Brown, is an important part of the tropical flora, which can be found mainly in Africa, Sou-theast Asia, and Australia. The family itself has 50 species and has not yet been studied very closely, except for the genus Sesamum and its representative Sesamum Indicum L., which is among the most important crops for food purposes. In this work, five representatives of the Pedaliaceae family were analyzed using the DNA barcoding method, using nuclear and chlo-roplast DNA regions, which served as a marker for their subsequent phylogenetic analysis and taxonomic classification. DNA isolation from plant samples, PCR, and DNA sequencing was performed to obtain plant DNA and the necessary results.
|
|
|
Současné metody sekvenování nukleových kyselin
Navrátil, Jiří
The Bachelor´s thesis deals with the description of the techniques of nucleic acids sequencing. Based on an analysis and synthesis of findings in scientific resources, the characteristics of the methods, a brief history, advantages and disadvantages, possible modifications and future applications are described in the theoretical section, whereas special reference is placed on the principle on which these methods work. A special cha-pter is devoted to the Human Genome Project, which is considered the greatest milestone in the history of sequencing. In the practical section, animal genome of order Lepidoptera was sequenced. The entire process included DNA extraction, its purification, amplification, evaluation of quality on an agarose gel, sequencing and, last but not least, an analysis of the output data using specialized software.
|
|
|
Evaluation of numerical representations suitability for overlap detection
Pleskačová, Barbora ; Maděránková, Denisa (referee) ; Jugas, Robin (advisor)
The bachelor´s thesis is focused on the evaluation of numerical representations suitability for overlap detection. Introductory part deals with description of deoxyribonucleic acid structure. The next part discribes sequencing methods and genome assembly techniques. Following part deals with numerical representations that convert DNA sequences into numerical form. Based on similarity metrics, the use of these representations is tested for the detection of overlaps between DNA reads. In the practical part of the thesis an algorithm for overlap detection is designed and implemented using numerical representations. The algorithm is then tested on data.
|
guest ::
