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Synthesis and analysis of quantum dot conjugates intended for fret sensor
 Datinská, Vladimíra ; Klepárník, Karel ; Belšánová, Barbora ; Minárik, M. ; Foret, František
Our work is focused on synthesis of sensor based on Fӧrster resonance energy transfer (FRET), which is aimed to be used for DNA mutation detection. The sensor is based on the attachment of laboratory synthesized quantum dot (QD) to ssDNA. A complementary chain of a sample is labeled by a fluorescent dye. Thus, only if dsDNA between both complementary chains is formed, the energy from QD (donor) can be transferr ed to the dye acceptor) and FRET is observed.
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