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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Microarray Data Analysis
 Vogel, Ivan ; Smrž, Pavel (referee) ; Šilhavá, Jana (advisor)
The aim of this bachelor thesis is to describe the DNA microarray technology. Several analytical algorithms are discussed. It describes closely the clustering methods, especially several hierarchical clustering modifications. It gives a summary of available commercial and uncommercial software for microarray analysis. The thesis describes own implementation design of hierarchical methods. At the end, results are compared with similar uncommercial software.
Detailed record
Genom analysis of \kur{Pseudomonas syringae} pathovars and design of suitable oligonucleotides for design of DNA chip
 BERAN, Pavel
Pseudomonas syringae van Hall 1902 (Ps) is important bacterial pathogen parasiting on wide spectrum of plants. Ps is sorted to pathovars according to host plant. The aim of this thesis was determining of genome size using restriction cleavage and PFGE on available Ps pathovars, sequencing of selected genes and oligonucleotide design for assembling DNA microarray. Genome sizes determined by restriction cleavage and separation with PFGE were different from database data by about 500 {--} 2000 kbp. Therefore the method is less usefull for accurate determining of genome size. Genes for 16S and 23S rRNA, 16S-23S ITS and gyrB were sequenced mutual variability of Ps pathovars was evaluated and compared to similar works. DNA sequencing was also basis for oligonucleotide DNA chip design.
Detailed record
Microarray Data Analysis
 Vogel, Ivan ; Smrž, Pavel (referee) ; Šilhavá, Jana (advisor)
The aim of this bachelor thesis is to describe the DNA microarray technology. Several analytical algorithms are discussed. It describes closely the clustering methods, especially several hierarchical clustering modifications. It gives a summary of available commercial and uncommercial software for microarray analysis. The thesis describes own implementation design of hierarchical methods. At the end, results are compared with similar uncommercial software.
Detailed record

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