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Impedance Spectroscope
Sliž, Jiří ; Pikula, Stanislav (referee) ; Beneš, Petr (advisor)
The aim of this thesis is to design a measuring instrument intended for impedance spectroscopy. There are definitions of the terms impedance and impedance spectroscopy and methods of its measurement. The integrated circuit AD5933 from Analog Devices was used for measurement. The measuring device is controlled by a microcontroller PIC32MX460F512L, which communicates with the measuring circuit, controls the actual measurement and communication with the control PC via the USB interface. The work also includes the creation of the control program, which ensures calibration, measurement, storage data, and data visualization. Device has also the possibility of repeated measurements and automatic switching of multiple inputs. Finally, the proposed device has been tested on two microbiological experiments. Impedance change depending on the concentration of bacteria in the measured solution was proved in first experiment. The aim of the second experiment is to distinguish bacterial strains of Staphylococcus epidermidis which creates biofilm and which not. With PLS was created validation model that can be used as a detector strains forming biofilm.
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Impedance Spectroscope
Sliž, Jiří ; Pikula, Stanislav (referee) ; Beneš, Petr (advisor)
The aim of this thesis is to design a measuring instrument intended for impedance spectroscopy. There are definitions of the terms impedance and impedance spectroscopy and methods of its measurement. The integrated circuit AD5933 from Analog Devices was used for measurement. The measuring device is controlled by a microcontroller PIC32MX460F512L, which communicates with the measuring circuit, controls the actual measurement and communication with the control PC via the USB interface. The work also includes the creation of the control program, which ensures calibration, measurement, storage data, and data visualization. Device has also the possibility of repeated measurements and automatic switching of multiple inputs. Finally, the proposed device has been tested on two microbiological experiments. Impedance change depending on the concentration of bacteria in the measured solution was proved in first experiment. The aim of the second experiment is to distinguish bacterial strains of Staphylococcus epidermidis which creates biofilm and which not. With PLS was created validation model that can be used as a detector strains forming biofilm.
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